Recombinant
RabMAb

Recombinant Anti-NPY5R antibody [EPR8382] (ab133757)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8382] to NPY5R
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NPY5R antibody [EPR8382]
    See all NPY5R primary antibodies

  • Description

    Rabbit monoclonal [EPR8382] to NPY5R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NPY5R aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q15761

  • Positive control

    • Human hypothalamus lysate, Human fetal brain lysate, Ramos cell lysate, U87 MG cells and cell lysate, Caco2 cell lysate, BxPC 3 cells and cell lysate.

  • General notes

    This product was previously labelled as Y5 receptor

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.

    • Tissue specificity

      Brain; hypothalamus.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Neuropeptide Y receptor type 5 antibody
      • Neuropeptide Y receptor Y5 antibody
      • NPY 5R antibody
      • NPY R5 antibody
      • NPY Y5 antibody
      • NPY Y5 receptor antibody
      • NPY-Y5 receptor antibody
      • NPY5 R antibody
      • NPY5-R antibody
      • NPY5R antibody
      • NPY5R_HUMAN antibody
      • NPYR 5 antibody
      • NPYR5 antibody
      • NPYY 5 antibody
      • NPYY5 antibody
      • NPYY5-R antibody
      • Y5 receptor antibody
      see all

    Images

    • Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)
      Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)
      All lanes : Anti-NPY5R antibody [EPR8382] (ab133757) at 1/10000 dilution

      Lane 1 : Human hypothalamus lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : U87 MG cell lysate
      Lane 5 : Caco 2 cell lysate
      Lane 6 : BxPC 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 51 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)
      Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)

      Immunofluorescent staining of BxPC3 cells labelling NPY5R with ab133757 at 1/250.

    • Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)
      Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)

      Immunofluorescent staining of U87 MG cells labelling NPY5R with ab133757 at 1/250.

    • Flow Cytometry - Anti-NPY5R antibody [EPR8382] (ab133757)
      Flow Cytometry - Anti-NPY5R antibody [EPR8382] (ab133757)
      Overlay histogram showing SHSY-5Y cells stained with ab133757 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133757, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Other - Anti-NPY5R antibody [EPR8382] (ab133757)
      Other - Anti-NPY5R antibody [EPR8382] (ab133757)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    Publishing research using ab133757? Please let us know so that we can cite the reference in this datasheet.

    ab133757 has been referenced in 1 publication.

    • Boudjadi S  et al. Involvement of the Integrin a1ß1 in the Progression of Colorectal Cancer. Cancers (Basel) 9:N/A (2017). PubMed: 28933766

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NPY5R antibody [EPR8382]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with tween
    Specification
    Breast cancer
    Fixative
    Paraformaldehyde
    Read More

    Mr. Rudolf Jung

    Verified customer

    Submitted Aug 01 2016

    Western blot abreview for Anti-NPY5R antibody [EPR8382]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Hypothalamus)
    Gel Running Conditions
    Reduced Denaturing (Tris-acetate 7%)
    Loading amount
    20 µg
    Specification
    Hypothalamus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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