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    npy5r-antibody-epr8382-ab133757.pdf

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RecombinantRabMAb

Recombinant Anti-NPY5R antibody [EPR8382] (ab133757)

  • Datasheet
Reviews (2) Submit a question References (2)

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Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)
  • Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)
  • Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)
  • Flow Cytometry (Intracellular) - Anti-NPY5R antibody [EPR8382] (ab133757)
  • OI-RD Scanning - Anti-NPY5R antibody [EPR8382] (ab133757)
  • Anti-NPY5R antibody [EPR8382] (ab133757)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8382] to NPY5R
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-NPY5R antibody [EPR8382]
    See all NPY5R primary antibodies
  • Description

    Rabbit monoclonal [EPR8382] to NPY5R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human NPY5R aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q15761

  • Positive control

    • Human hypothalamus lysate, Human fetal brain lysate, Ramos cell lysate, U87 MG cells and cell lysate, Caco2 cell lysate, BxPC 3 cells and cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.80 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8382
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • More Neuropeptides
    • Neuroscience
    • Neurology process
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-NPY5R antibody [EPR8382] - BSA and Azide free (ab248642)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human NPY5R protein (ab132113)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
ICC/IF
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
  • Tissue specificity

    Brain; hypothalamus.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q15761 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4889 Human
    • Entrez Gene: 18168 Mouse
    • Entrez Gene: 25340 Rat
    • Omim: 602001 Human
    • SwissProt: Q15761 Human
    • SwissProt: O70342 Mouse
    • SwissProt: Q63634 Rat
    • Unigene: 519058 Human
    • Unigene: 598503 Human
    • Unigene: 10685 Mouse
    • Unigene: 10532 Rat
    see all
  • Alternative names

    • Neuropeptide Y receptor type 5 antibody
    • Neuropeptide Y receptor Y5 antibody
    • NPY 5R antibody
    • NPY R5 antibody
    • NPY Y5 antibody
    • NPY Y5 receptor antibody
    • NPY-Y5 receptor antibody
    • NPY5 R antibody
    • NPY5-R antibody
    • NPY5R antibody
    • NPY5R_HUMAN antibody
    • NPYR 5 antibody
    • NPYR5 antibody
    • NPYY 5 antibody
    • NPYY5 antibody
    • NPYY5-R antibody
    • Y5 receptor antibody
    see all

Images

  • Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)
    Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)
    All lanes : Anti-NPY5R antibody [EPR8382] (ab133757) at 1/10000 dilution

    Lane 1 : Human hypothalamus lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : U87 MG cell lysate
    Lane 5 : Caco 2 cell lysate
    Lane 6 : BxPC 3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)
    Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)

    Immunofluorescent staining of BxPC3 cells labelling NPY5R with ab133757 at 1/250.

  • Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)
    Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)

    Immunofluorescent staining of U87 MG cells labelling NPY5R with ab133757 at 1/250.

  • Flow Cytometry (Intracellular) - Anti-NPY5R antibody [EPR8382] (ab133757)
    Flow Cytometry (Intracellular) - Anti-NPY5R antibody [EPR8382] (ab133757)

    Overlay histogram showing SHSY-5Y cells stained with ab133757 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133757, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

     

  • OI-RD Scanning - Anti-NPY5R antibody [EPR8382] (ab133757)
    OI-RD Scanning - Anti-NPY5R antibody [EPR8382] (ab133757)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NPY5R antibody [EPR8382] (ab133757)
    Anti-NPY5R antibody [EPR8382] (ab133757)

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab133757? Please let us know so that we can cite the reference in this datasheet.

ab133757 has been referenced in 2 publications.

  • Abualsaud N  et al. Neuropeptide Y/Y5 Receptor Pathway Stimulates Neuroblastoma Cell Motility Through RhoA Activation. Front Cell Dev Biol 8:627090 (2020). PubMed: 33681186
  • Boudjadi S  et al. Involvement of the Integrin a1ß1 in the Progression of Colorectal Cancer. Cancers (Basel) 9:N/A (2017). PubMed: 28933766

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NPY5R antibody [EPR8382]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with tween
Specification
Breast cancer
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Rudolf Jung

Verified customer

Submitted Aug 01 2016

Western blot abreview for Anti-NPY5R antibody [EPR8382]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Hypothalamus)
Gel Running Conditions
Reduced Denaturing (Tris-acetate 7%)
Loading amount
20 µg
Specification
Hypothalamus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 15 2016

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