Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8382] to NPY5R
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-NPY5R antibody [EPR8382]
See all NPY5R primary antibodies
DescriptionRabbit monoclonal [EPR8382] to NPY5R
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NPY5R aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: Q15761
- Human hypothalamus lysate, Human fetal brain lysate, Ramos cell lysate, U87 MG cells and cell lysate, Caco2 cell lysate, BxPC 3 cells and cell lysate.
This product was previously labelled as Y5 receptor
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.80 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionReceptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
Tissue specificityBrain; hypothalamus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- Neuropeptide Y receptor type 5 antibody
- Neuropeptide Y receptor Y5 antibody
- NPY 5R antibody
All lanes : Anti-NPY5R antibody [EPR8382] (ab133757) at 1/10000 dilution
Lane 1 : Human hypothalamus lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Ramos cell lysate
Lane 4 : U87 MG cell lysate
Lane 5 : Caco 2 cell lysate
Lane 6 : BxPC 3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 51 kDa
Immunofluorescent staining of BxPC3 cells labelling NPY5R with ab133757 at 1/250.
Immunofluorescent staining of U87 MG cells labelling NPY5R with ab133757 at 1/250.
Overlay histogram showing SHSY-5Y cells stained with ab133757 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133757, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133757 has been referenced in 1 publication.
- Boudjadi S et al. Involvement of the Integrin a1ß1 in the Progression of Colorectal Cancer. Cancers (Basel) 9:N/A (2017). PubMed: 28933766