Recombinant Anti-NPY5R antibody [EPR8382] (ab133757)
![Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)](https://www.abcam.com/ps/products/133/ab133757/Images/NPY5R-Primary-antibodies-ab133757-3.jpg "Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8382] to NPY5R
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NPY5R antibody [EPR8382]
See all NPY5R primary antibodies -
Description
Rabbit monoclonal [EPR8382] to NPY5R -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human NPY5R aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: Q15761 -
Positive control
- Human hypothalamus lysate, Human fetal brain lysate, Ramos cell lysate, U87 MG cells and cell lysate, Caco2 cell lysate, BxPC 3 cells and cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8382 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
|
| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). |
|
ICC/IF
1/250 - 1/500. |
Target
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Function
Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders. -
Tissue specificity
Brain; hypothalamus. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4889 Human
- Entrez Gene: 18168 Mouse
- Entrez Gene: 25340 Rat
- Omim: 602001 Human
- SwissProt: Q15761 Human
- SwissProt: O70342 Mouse
- SwissProt: Q63634 Rat
- Unigene: 519058 Human
see all -
Alternative names
- Neuropeptide Y receptor type 5 antibody
- Neuropeptide Y receptor Y5 antibody
- NPY 5R antibody
see all
Images
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Western blot - Anti-NPY5R antibody [EPR8382] (ab133757)All lanes : Anti-NPY5R antibody [EPR8382] (ab133757) at 1/10000 dilution
Lane 1 : Human hypothalamus lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Ramos cell lysate
Lane 4 : U87 MG cell lysate
Lane 5 : Caco 2 cell lysate
Lane 6 : BxPC 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 51 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)](https://www.abcam.com/ps/products/133/ab133757/Images/NPY5R-Primary-antibodies-ab133757-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)Immunofluorescent staining of BxPC3 cells labelling NPY5R with ab133757 at 1/250.
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![Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)](https://www.abcam.com/ps/products/133/ab133757/Images/ab133757-147856-anti-y5-receptor-antibody-epr8382-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NPY5R antibody [EPR8382] (ab133757)Immunofluorescent staining of U87 MG cells labelling NPY5R with ab133757 at 1/250.
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![Flow Cytometry (Intracellular) - Anti-NPY5R antibody [EPR8382] (ab133757)](https://www.abcam.com/ps/products/133/ab133757/Images/ab133757-5-ab133757FC.jpg)
Flow Cytometry (Intracellular) - Anti-NPY5R antibody [EPR8382] (ab133757)Overlay histogram showing SHSY-5Y cells stained with ab133757 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133757, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![OI-RD Scanning - Anti-NPY5R antibody [EPR8382] (ab133757)](https://www.abcam.com/ps/products/133/ab133757/Images/NPY5R-Primary-antibodies-ab133757-4.jpg)
OI-RD Scanning - Anti-NPY5R antibody [EPR8382] (ab133757) -
![Anti-NPY5R antibody [EPR8382] (ab133757)](https://www.abcam.com/ps/products/133/ab133757/Images/ab133757-6-benefits-of-recombinant-antibodies.png)
Anti-NPY5R antibody [EPR8382] (ab133757)
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab133757 has been referenced in 2 publications.
- Abualsaud N et al. Neuropeptide Y/Y5 Receptor Pathway Stimulates Neuroblastoma Cell Motility Through RhoA Activation. Front Cell Dev Biol 8:627090 (2020). PubMed: 33681186
- Boudjadi S et al. Involvement of the Integrin a1ß1 in the Progression of Colorectal Cancer. Cancers (Basel) 9:N/A (2017). PubMed: 28933766
