Overview

  • Product name

    Anti-NPY5R antibody [EPR8382]
    See all NPY5R primary antibodies
  • Description

    Rabbit monoclonal [EPR8382] to NPY5R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NPY5R aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q15761

  • Positive control

    • Human hypothalamus lysate, Human fetal brain lysate, Ramos cell lysate, U87 MG cells and cell lysate, Caco2 cell lysate, BxPC 3 cells and cell lysate.
  • General notes

    This product was previously labelled as Y5 receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
    • Tissue specificity

      Brain; hypothalamus.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Neuropeptide Y receptor type 5 antibody
      • Neuropeptide Y receptor Y5 antibody
      • NPY 5R antibody
      • NPY R5 antibody
      • NPY Y5 antibody
      • NPY Y5 receptor antibody
      • NPY-Y5 receptor antibody
      • NPY5 R antibody
      • NPY5-R antibody
      • NPY5R antibody
      • NPY5R_HUMAN antibody
      • NPYR 5 antibody
      • NPYR5 antibody
      • NPYY 5 antibody
      • NPYY5 antibody
      • NPYY5-R antibody
      • Y5 receptor antibody
      see all

    Images

    • All lanes : Anti-NPY5R antibody [EPR8382] (ab133757) at 1/10000 dilution

      Lane 1 : Human hypothalamus lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : U87 MG cell lysate
      Lane 5 : Caco 2 cell lysate
      Lane 6 : BxPC 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 51 kDa

    • Immunofluorescent staining of BxPC3 cells labelling NPY5R with ab133757 at 1/250.

    • Immunofluorescent staining of U87 MG cells labelling NPY5R with ab133757 at 1/250.

    • Overlay histogram showing SHSY-5Y cells stained with ab133757 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133757, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with tween
    Specification
    Breast cancer
    Fixative
    Paraformaldehyde

    Mr. Rudolf Jung

    Verified customer

    Submitted Aug 01 2016

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Hypothalamus)
    Gel Running Conditions
    Reduced Denaturing (Tris-acetate 7%)
    Loading amount
    20 µg
    Specification
    Hypothalamus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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