Overview

  • Product name
    NQO1 Activity Assay Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3 2.183%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9 6.035%
  • Sample type
    Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate
  • Assay type
    Enzyme activity
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.


    The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm. Dicoumarol is used as an inhibitor of NQO1 as part of the assay.


    NQO1 assay protocol summary:
    - add samples to wells
    - add reaction buffer + inhibitor and reaction buffer to separate sample wells
    - analyze with a microplate reader for 5 min

  • Notes

    NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

  • Platform
    Microplate reader

Properties

Images

  • Figure 1

  • Figure 2. Raw data is expressed as rate (mOD/min) per microgram of cell extract added per well as shown above.

  • Figure 3a

  • Figure 3b

  • Figure 3c

  • Figure 4

Protocols

References

This product has been referenced in:
  • Ling Y  et al. Development of novel amino-quinoline-5,8-dione derivatives as NAD(P)H:quinone oxidoreductase 1 (NQO1) inhibitors with potent antiproliferative activities. Eur J Med Chem 154:199-209 (2018). Read more (PubMed: 29803003) »
  • Madajewski B  et al. Depleting Tumor-NQO1 Potentiates Anoikis and Inhibits Growth of NSCLC. Mol Cancer Res 14:14-25 (2016). Read more (PubMed: 26553038) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer


I am sorry we do not cell HepG2 tissue lysate prepared using a suitable method for use with ab184867 NQO1 Activity Assay Kit. The lysates we sell are prepared for western blotting.

Sorry I am not able to provide a more positive answer on this occasion.

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