Anti-NQO1 antibody (ab97385)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-NQO1 antibody
See all NQO1 primary antibodies -
Description
Rabbit polyclonal to NQO1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide contain a sequence corresponding to a region within amino acids 213 and 224 of Human NQO1 9(NP_000894).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates for WB; HeLa cells for IF; CL1-0 xenograft for IHC-P.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 31 kDa. In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1728 Human
- Omim: 125860 Human
- SwissProt: P15559 Human
- Unigene: 406515 Human
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Alternative names
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
Images
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Western blot - Anti-NQO1 antibody (ab97385)All lanes : Anti-NQO1 antibody (ab97385) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab97385)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97385 at 1/200 dilution. Lower image merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab97385 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)Image from Hevir N et al., Molecular and Cellular Endocrinology, Volume 367, Issue1-2, March 2013, Pages 74-84. Fig 4.; doi: 10.1016/j.mce.2012.12.019. Epub 2012 Dec 28 with permission from Elsevier.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian endometriosis tissue, staining NQO1 with ab97385.
Antigen retrieval was performed in sodium citrate buffer in a microwave oven. Samples were blocked with rabbit serum for one hour before incubating overnight with primary antibody (1/200). Stainined was detected using DAB.
Protocols
References
This product has been referenced in:
- Ravasz D et al. Reduction of 2-methoxy-1,4-naphtoquinone by mitochondrially-localized Nqo1 yielding NAD+ supports substrate-level phosphorylation during respiratory inhibition. Biochim Biophys Acta N/A:N/A (2018). WB . Read more (PubMed: 29746824) »
- Hevir N et al. Disturbed balance between phase I and II metabolizing enzymes in ovarian endometriosis: A source of excessive hydroxy-estrogens and ROS? Mol Cell Endocrinol : (2012). WB, IHC-P ; Human . Read more (PubMed: 23277161) »
