Overview

  • Product name
    Anti-NQO1 antibody
    See all NQO1 primary antibodies
  • Description
    Rabbit polyclonal to NQO1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide contain a sequence corresponding to a region within amino acids 213 and 224 of Human NQO1 9(NP_000894).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates for WB; HeLa cells for IF; CL1-0 xenograft for IHC-P.

Properties

Applications

Our Abpromise guarantee covers the use of ab97385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa. In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similarities
    Belongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • Azoreductase antibody
      • Cytochrome b 5 reductase antibody
      • DHQU antibody
      • DIA 4 antibody
      • DIA4 antibody
      • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
      • Diaphorase (NADH/NADPH) antibody
      • Diaphorase 4 antibody
      • Dioxin inducible 1 antibody
      • DT diaphorase antibody
      • DT-diaphorase antibody
      • DTD antibody
      • Menadione reductase antibody
      • NAD(P)H dehydrogenase [quinone] 1 antibody
      • NAD(P)H dehydrogenase quinone 1 antibody
      • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
      • NAD(P)H quinone dehydrogenase 1 antibody
      • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
      • NAD(P)H:menadione oxidoreductase 1 antibody
      • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
      • NAD(P)H:quinone oxidoreductase 1 antibody
      • NAD(P)H:quinone oxireductase antibody
      • NMOR 1 antibody
      • NMOR I antibody
      • NMOR1 antibody
      • NMORI antibody
      • NQO 1 antibody
      • NQO1 antibody
      • NQO1_HUMAN antibody
      • Phylloquinone reductase antibody
      • QR 1 antibody
      • QR1 antibody
      • Quinone reductase 1 antibody
      see all

    Images

    • Western blot - Anti-NQO1 antibody (ab97385)
      Western blot - Anti-NQO1 antibody (ab97385)
      All lanes : Anti-NQO1 antibody (ab97385) at 1/1000 dilution

      Lane 1 : MOLT4 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 31 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab97385)
      Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab97385)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97385 at 1/200 dilution. Lower image merged with DNA probe.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)
      Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab97385 at 1/500 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (ab97385)Image from Hevir N et al., Molecular and Cellular Endocrinology, Volume 367, Issue1-2, March 2013, Pages 74-84. Fig 4.; doi: 10.1016/j.mce.2012.12.019. Epub 2012 Dec 28 with permission from Elsevier.

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian endometriosis tissue, staining NQO1 with ab97385.

      Antigen retrieval was performed in sodium citrate buffer in a microwave oven. Samples were blocked with rabbit serum for one hour before incubating overnight with primary antibody (1/200). Stainined was detected using DAB.

    References

    This product has been referenced in:
    • Ravasz D  et al. Reduction of 2-methoxy-1,4-naphtoquinone by mitochondrially-localized Nqo1 yielding NAD+ supports substrate-level phosphorylation during respiratory inhibition. Biochim Biophys Acta N/A:N/A (2018). WB . Read more (PubMed: 29746824) »
    • Hevir N  et al. Disturbed balance between phase I and II metabolizing enzymes in ovarian endometriosis: A source of excessive hydroxy-estrogens and ROS? Mol Cell Endocrinol : (2012). WB, IHC-P ; Human . Read more (PubMed: 23277161) »

    See all 2 Publications for this product

    Publishing research using ab97385? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NQO1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (ovarian endometriosis)
    Specification
    ovarian endometriosis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH=6) in microwave oven
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted May 17 2012

    Western blot abreview for Anti-NQO1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (endometriosis, normal endometrium)
    Loading amount
    30 µg
    Specification
    endometriosis, normal endometrium
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 16 2012

    I’ve tested the antibody NQO1 (ab97385) which you send as a replacement for the previous one and I’m still not satisfied with the results. I attached a full report on my experiments so that you can revise it carefully and let me know if I did somethin...

    Read More

    Reply from the lab and the image is attached: "Our QC image also shows there are double bands in HepG2 cell line, and the pattern of these two bands are similar with the double bands with ~31KDa and ~26KDa detected in HepG2 by the customer. We bel...

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