Anti-NQO1 antibody - C-terminal (ab219769)

Goat polyclonal NQO1 antibody. Validated in WB, IHC and tested in Rat, Human, Pig. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-NQO1 antibody - C-terminal
    See all NQO1 primary antibodies
  • Description

    Goat polyclonal to NQO1 - C-terminal
  • Host species

    Goat
  • Specificity

    ab219769 is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human, Pig
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog, Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human NQO1 aa 263-274 (C terminal) (Cysteine residue). (NP_000894.1).
    Sequence:

    SIPTDNQIKARK


    Database link: P15559

  • Positive control

    • Human kidney tissue; human, rat and pig kidney lysates, human lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab219769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similarities

    Belongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Azoreductase antibody
    • Cytochrome b 5 reductase antibody
    • DHQU antibody
    • DIA 4 antibody
    • DIA4 antibody
    • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
    • Diaphorase (NADH/NADPH) antibody
    • Diaphorase 4 antibody
    • Dioxin inducible 1 antibody
    • DT diaphorase antibody
    • DT-diaphorase antibody
    • DTD antibody
    • Menadione reductase antibody
    • NAD(P)H dehydrogenase [quinone] 1 antibody
    • NAD(P)H dehydrogenase quinone 1 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
    • NAD(P)H quinone dehydrogenase 1 antibody
    • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
    • NAD(P)H:menadione oxidoreductase 1 antibody
    • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
    • NAD(P)H:quinone oxidoreductase 1 antibody
    • NAD(P)H:quinone oxireductase antibody
    • NMOR 1 antibody
    • NMOR I antibody
    • NMOR1 antibody
    • NMORI antibody
    • NQO 1 antibody
    • NQO1 antibody
    • NQO1_HUMAN antibody
    • Phylloquinone reductase antibody
    • QR 1 antibody
    • QR1 antibody
    • Quinone reductase 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling NQO1 with ab219769 at 5 µg/ml.

  • All lanes : Anti-NQO1 antibody - C-terminal (ab219769) at 1 µg/ml

    Lane 1 : Rat kidney lysate (in RIPA buffer)
    Lane 2 : Pig kidney lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Anti-NQO1 antibody - C-terminal (ab219769) at 0.03 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Anti-NQO1 antibody - C-terminal (ab219769) at 1 µg/ml + Human lung lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa

References

ab219769 has not yet been referenced specifically in any publications.

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