Product nameAnti-NQO1 antibody [EPR21230]
See all NQO1 primary antibodies
DescriptionRabbit monoclonal [EPR21230] to NQO1
SpecificityBased on internal western blot testing, this antibody showed no reactivity to NQO1 in mouse samples.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Pig
Database link: P15559
- WB: HAP1, HepG2, Human Kidney
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Our Abpromise guarantee covers the use of ab213239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Abcam recommends using 3% Milk as a blocking agent.
FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
- Information by UniProt
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
All lanes : Anti-NQO1 antibody [EPR21230] (ab213239) at 1 µg/ml
Lane 1 : HAP1 wild-type whole cell lysate at 40 µg
Lane 2 : NQO1 knockout whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Lane 4 : MEF1 whole cell lysate at 20 µg
Lane 5 : Human Kidney tissue lysate at 20 µg
Lane 6 : Mouse Kidney tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 31 kDa
ab213239 was shown to specifically react with NQO1 in wild type HAP1 cells. No band was observed when NQO1 knockout samples were used. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. The membrane was then blocked for an hour using 3% milk before being incubated with ab213239 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a concentration of 1ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab213239 has not yet been referenced specifically in any publications.