Overview

  • Product name

    Anti-NQO1 antibody [EPR21230]
    See all NQO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21230] to NQO1
  • Host species

    Rabbit
  • Specificity

    Based on internal western blot testing, this antibody showed no reactivity to NQO1 in mouse samples.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment.
    Database link: P15559

  • Positive control

    • WB: HAP1, HepG2, Human Kidney
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

Applications

Our Abpromise guarantee covers the use of ab213239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Abcam recommends using 3% Milk as a blocking agent.

Target

  • Function

    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similarities

    Belongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Azoreductase antibody
    • Cytochrome b 5 reductase antibody
    • DHQU antibody
    • DIA 4 antibody
    • DIA4 antibody
    • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
    • Diaphorase (NADH/NADPH) antibody
    • Diaphorase 4 antibody
    • Dioxin inducible 1 antibody
    • DT diaphorase antibody
    • DT-diaphorase antibody
    • DTD antibody
    • Menadione reductase antibody
    • NAD(P)H dehydrogenase [quinone] 1 antibody
    • NAD(P)H dehydrogenase quinone 1 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
    • NAD(P)H quinone dehydrogenase 1 antibody
    • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
    • NAD(P)H:menadione oxidoreductase 1 antibody
    • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
    • NAD(P)H:quinone oxidoreductase 1 antibody
    • NAD(P)H:quinone oxireductase antibody
    • NMOR 1 antibody
    • NMOR I antibody
    • NMOR1 antibody
    • NMORI antibody
    • NQO 1 antibody
    • NQO1 antibody
    • NQO1_HUMAN antibody
    • Phylloquinone reductase antibody
    • QR 1 antibody
    • QR1 antibody
    • Quinone reductase 1 antibody
    see all

Images

  • All lanes : Anti-NQO1 antibody [EPR21230] (ab213239) at 1 µg/ml

    Lane 1 : HAP1 wild-type whole cell lysate at 40 µg
    Lane 2 : NQO1 knockout whole cell lysate at 40 µg
    Lane 3 : HepG2 whole cell lysate at 20 µg
    Lane 4 : MEF1 whole cell lysate at 20 µg
    Lane 5 : Human Kidney tissue lysate at 20 µg
    Lane 6 : Mouse Kidney tissue lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 31 kDa



    ab213239 was shown to specifically react with NQO1 in wild type HAP1 cells. No band was observed when NQO1 knockout samples were used. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. The membrane was then blocked for an hour using 3% milk before being incubated with ab213239 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a concentration of 1ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

     

     

References

ab213239 has not yet been referenced specifically in any publications.

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