Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21230] to NQO1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Product nameAnti-NQO1 antibody [EPR21230]
See all NQO1 primary antibodies
DescriptionRabbit monoclonal [EPR21230] to NQO1
SpecificityBased on internal western blot testing, this antibody showed no reactivity to NQO1 in mouse samples.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Pig
Database link: P15559
- WB: HAP1, HepG2, Human Kidney
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Our Abpromise guarantee covers the use of ab213239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Abcam recommends using 3% Milk as a blocking agent.
FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
- Information by UniProt
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
All lanes : Anti-NQO1 antibody [EPR21230] (ab213239) at 1 µg/ml
Lane 1 : HAP1 wild-type whole cell lysate at 40 µg
Lane 2 : NQO1 knockout whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Lane 4 : MEF1 whole cell lysate at 20 µg
Lane 5 : Human Kidney tissue lysate at 20 µg
Lane 6 : Mouse Kidney tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 31 kDa
ab213239 was shown to specifically react with NQO1 in wild type HAP1 cells. No band was observed when NQO1 knockout samples were used. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. The membrane was then blocked for an hour using 3% milk before being incubated with ab213239 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a concentration of 1ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab213239 has not yet been referenced specifically in any publications.