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  1. Link

    nqo1-antibody-epr3309-ab80588.pdf

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Signal Transduction Metabolism Drug metabolism
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NQO1 antibody [EPR3309] (ab80588)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (61)

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Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
  • Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588)
  • Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588)
  • Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)
  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
  • Anti-NQO1 antibody [EPR3309] (ab80588)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3309] to NQO1
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

Primary
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Anti-Nrf2 antibody [EP1808Y] - BSA and Azide free (ab180845)
Assay
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NQO1 Activity Assay Kit (ab184867)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NQO1 antibody [EPR3309]
    See all NQO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3309] to NQO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NQO1 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y, MCF7, HeLa and A549 cell lysates, rat brain tissue lysate. ICC/IF: MCF-7 cells. IP: HeLa cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3309
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-NQO1 antibody [EPR3309] (ab196196)
    • Alexa Fluor® 488 Anti-NQO1 antibody [EPR3309] (ab196465)
    • Anti-NQO1 antibody [EPR3309] - BSA and Azide free (ab239896)
  • Assay kits

    • NQO1 Activity Assay Kit (ab184867)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human NQO1 protein (ab87692)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab80588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB (4)
1/10000 - 1/150000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP
1/30 - 1/50.

 

ICC/IF
1/50 - 1/80.

 

Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/10000 - 1/150000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP
1/30 - 1/50.

 

ICC/IF
1/50 - 1/80.

 

Target

  • Function

    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similarities

    Belongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P15559 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1728 Human
    • Entrez Gene: 18104 Mouse
    • Entrez Gene: 24314 Rat
    • Omim: 125860 Human
    • SwissProt: P15559 Human
    • SwissProt: Q64669 Mouse
    • SwissProt: P05982 Rat
    • Unigene: 406515 Human
    • Unigene: 252 Mouse
    • Unigene: 11234 Rat
    see all
  • Alternative names

    • Azoreductase antibody
    • Cytochrome b 5 reductase antibody
    • DHQU antibody
    • DIA 4 antibody
    • DIA4 antibody
    • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
    • Diaphorase (NADH/NADPH) antibody
    • Diaphorase 4 antibody
    • Dioxin inducible 1 antibody
    • DT diaphorase antibody
    • DT-diaphorase antibody
    • DTD antibody
    • Menadione reductase antibody
    • NAD(P)H dehydrogenase [quinone] 1 antibody
    • NAD(P)H dehydrogenase quinone 1 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
    • NAD(P)H quinone dehydrogenase 1 antibody
    • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
    • NAD(P)H:menadione oxidoreductase 1 antibody
    • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
    • NAD(P)H:quinone oxidoreductase 1 antibody
    • NAD(P)H:quinone oxireductase antibody
    • NMOR 1 antibody
    • NMOR I antibody
    • NMOR1 antibody
    • NMORI antibody
    • NQO 1 antibody
    • NQO1 antibody
    • NQO1_HUMAN antibody
    • Phylloquinone reductase antibody
    • QR 1 antibody
    • QR1 antibody
    • Quinone reductase 1 antibody
    see all

Images

  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
    Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
    All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
    Lane 2 : NQO1 knockout HAP1 whole cell lysate at 40 µg
    Lane 3 : HepG2 whole cell lysate at 20 µg
    Lane 4 : MEF1 whole cell lysate at 20 µg
    Lane 5 : Human Kidney tissue lysate at 20 µg
    Lane 6 : Mouse Kidney tissue lysate at 20 µg

    Predicted band size: 31 kDa



    Lanes 1 - 6: Merged signal (red and green). Green - ab80588 observed at 31 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab80588 was shown to recognize NQO1 in wild-type HAP1 cells as signal was lost at the expected MW in NQO1 knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. Ab80588 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
    Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
    All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1/25000 dilution (purified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588)
    Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588)

    Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling NQO1 (green) with purified ab80588 at 1/80. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588)
    Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588)

    ab80588 staining NQO1in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)
    Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)

    ab80588 (purified) at 1/50 immunoprecipitating NQO1 in HeLa cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
    Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)
    All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1/50000 dilution (unpurified)

    Lane 1 : MCF7
    cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549
    cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Anti-NQO1 antibody [EPR3309] (ab80588)
    Anti-NQO1 antibody [EPR3309] (ab80588)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (61)

Publishing research using ab80588? Please let us know so that we can cite the reference in this datasheet.

ab80588 has been referenced in 61 publications.

  • Yao X  et al. Chondrocyte ferroptosis contribute to the progression of osteoarthritis. J Orthop Translat 27:33-43 (2021). PubMed: 33376672
  • Cao X  et al. ROS-mediated hypomethylation of PRDX5 promotes STAT3 binding and activates the Nrf2 signaling pathway in NSCLC. Int J Mol Med 47:573-582 (2021). PubMed: 33416106
  • Li R  et al. Acidic fibroblast growth factor attenuates type 2 diabetes-induced demyelination via suppressing oxidative stress damage. Cell Death Dis 12:107 (2021). PubMed: 33479232
  • Buitrago-Molina LE  et al. p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model. Cell Mol Gastroenterol Hepatol 11:1387-1404 (2021). PubMed: 33484913
  • Jia XB  et al. Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway. Biol Res 54:7 (2021). PubMed: 33653412
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-NQO1 antibody [EPR3309]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Oct 17 2019

Western blot abreview for Anti-NQO1 antibody [EPR3309]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
20 µg
Specification
RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 14 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NQO1 antibody [EPR3309]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB-468 cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
MDA-MB-468 cells
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 31 2018

Western blot abreview for Anti-NQO1 antibody [EPR3309]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (B16 tumor lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
B16 tumor lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 09 2018

Western blot abreview for Anti-NQO1 antibody [EPR3309]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-468 cell lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MDA-MB-468 cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 09 2018

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