Recombinant Anti-NQO1 antibody [EPR3310] (ab79694)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NQO1 antibody [EPR3310]
See all NQO1 primary antibodies -
Description
Rabbit monoclonal [EPR3310] to NQO1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NQO1 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- MCF-7, HeLa, A549 and HepG2 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3310 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). | |
| IP | 1/10. |
Target
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Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1728 Human
- Omim: 125860 Human
- SwissProt: P15559 Human
- Unigene: 406515 Human
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Alternative names
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
Images
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![Western blot - Anti-NQO1 antibody [EPR3310] (ab79694)](https://www.abcam.com/ps/products/79/ab79694/Images/NQO1-Primary-antibodies-ab79694-1.jpg)
Western blot - Anti-NQO1 antibody [EPR3310] (ab79694)All lanes : Anti-NQO1 antibody [EPR3310] (ab79694) at 1/2000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Datasheets and documents
References
ab79694 has not yet been referenced specifically in any publications.
