Overview

  • Product name

  • Description

    Rabbit polyclonal to NQO2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-231 of Human NQO2.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Mouse liver and Raji cell lysates; PLC xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similarities

    Belongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHQV antibody
    • DIA6 antibody
    • EC 1.10.99.2 antibody
    • MGC94180 antibody
    • NAD(P)H dehydrogenase quinone 2 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody
    • NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody
    • NMOR2 antibody
    • NQO 2 antibody
    • NQO2 antibody
    • NQO2_HUMAN antibody
    • NRH dehydrogenase [quinone] 2 antibody
    • NRH dehydrogenase antibody
    • NRH:quinone oxidoreductase 2 antibody
    • OTTHUMP00000015948 antibody
    • OTTHUMP00000015949 antibody
    • OTTHUMP00000015953 antibody
    • Ox 2 antibody
    • Ox2 antibody
    • QR2 antibody
    • Quinone antibody
    • Quinone reductase 2 antibody
    • Ribosyldihydronicotinamide dehydrogenase [quinone] antibody
    • Ribosyldihydronicotinamide dehydrogenase antibody
    see all

Images

  • All lanes : Anti-NQO2 antibody (ab137612) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-NQO2 antibody (ab137612) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • ab137612, at a 1/100 dilution, staining NQO2 in paraffin embedded PLC xenograft tissue by Immunohistochemistry.
  • Immunofluorescent staining of NQO2 in HeLa cells with ab137612 at a dilution of 1/500. The cells were fixed in 4% PFA for 15 minutes at room temperature and counter-stained with Hoechst 33342 (blue).

References

ab137612 has not yet been referenced specifically in any publications.

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