Key features and details
- Rabbit polyclonal to NQO2
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NQO2 antibody
See all NQO2 primary antibodies
DescriptionRabbit polyclonal to NQO2
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant full length protein corresponding to Human NQO2 aa 1-231.
MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPR ATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIF QFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTG GTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEE RKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ
Database link: P16083
- WB: K562 whole cell lysate; Mouse liver lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells. IP: K562 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 26 kDa.|
FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
- Information by UniProt
- DHQV antibody
- DIA6 antibody
- EC 220.127.116.11 antibody
All lanes : Anti-NQO2 antibody (ab236917) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Mouse liver lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Paraffin-embedded human brain tissue stained for NQO2 using ab236917 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NQO2 (green) using ab236917 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
NQO2 was immunoprecipitated from 500 µg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with ab236917 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in K562 whole cell lysate.
Lane 2: ab236917 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236917 has not yet been referenced specifically in any publications.