Product nameAnti-NQO2 antibody
See all NQO2 primary antibodies
DescriptionRabbit polyclonal to NQO2
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant full length protein corresponding to Human NQO2 aa 1-231.
MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPR ATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIF QFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTG GTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEE RKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ
Database link: P16083
- WB: K562 whole cell lysate; Mouse liver lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells. IP: K562 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 26 kDa.|
FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
- Information by UniProt
- DHQV antibody
- DIA6 antibody
- EC 22.214.171.124 antibody
All lanes : Anti-NQO2 antibody (ab236917) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Mouse liver lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Paraffin-embedded human brain tissue stained for NQO2 using ab236917 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NQO2 (green) using ab236917 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
NQO2 was immunoprecipitated from 500 µg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with ab236917 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in K562 whole cell lysate.
Lane 2: ab236917 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
ab236917 has not yet been referenced specifically in any publications.