Recombinant Anti-NQO2 antibody [EPR13345] (ab181049)
![Western blot - Anti-NQO2 antibody [EPR13345] (ab181049)](https://www.abcam.com/ps/products/181/ab181049/Images/ab181049-215207-anti-nqo2-antibody-epr13345-western-blot.jpg "Western blot - Anti-NQO2 antibody [EPR13345] (ab181049)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13345] to NQO2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-NQO2 antibody [EPR13345]
See all NQO2 primary antibodies -
Description
Rabbit monoclonal [EPR13345] to NQO2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse kidney, rat heart, rat kidney, rat spleen, L6 and C2C12 lysates; C6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13345 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/140.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/140. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4835 Human
- Entrez Gene: 18105 Mouse
- Entrez Gene: 291084 Rat
- Omim: 160998 Human
- SwissProt: P16083 Human
- SwissProt: Q9JI75 Mouse
- SwissProt: Q6AY80 Rat
- Unigene: 533050 Human
see all -
Alternative names
- DHQV antibody
- DIA6 antibody
- EC 1.10.99.2 antibody
see all
Images
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Western blot - Anti-NQO2 antibody [EPR13345] (ab181049)All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa -
![Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] (ab181049)](https://www.abcam.com/ps/products/181/ab181049/Images/ab181049-215208-anti-nqo2-antibody-epr13345-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] (ab181049)Intracellular flow cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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![Western blot - Anti-NQO2 antibody [EPR13345] (ab181049)](https://www.abcam.com/ps/products/181/ab181049/Images/ab181049-215206-anti-nqo2-antibody-epr13345-western-blot.jpg)
Western blot - Anti-NQO2 antibody [EPR13345] (ab181049)All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/5000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Anti-NQO2 antibody [EPR13345] (ab181049)](https://www.abcam.com/ps/products/181/ab181049/Images/ab181049-4-benefits-of-recombinant-antibodies.png)
Anti-NQO2 antibody [EPR13345] (ab181049)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181049 has not yet been referenced specifically in any publications.