Key features and details
- Rabbit polyclonal to NR0B1 / Dax1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NR0B1 / Dax1 antibody
See all NR0B1 / Dax1 primary antibodies
DescriptionRabbit polyclonal to NR0B1 / Dax1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment: APEATLGPCW GCSCGSDPGV GRAGLGGRPV ALLYRCCFCG EDHPRQGSIL YSLLTSSKQT HVAPAAPEAR PGGAWWDRSY FAQRPGGKEA LPGGRATALL YRCCFCGEDH PQQGSTLYCV PTSTNQAQAA PEERPRAPWW DTSSGALRPV ALKSPQVVCE AASAGLLKTL RFVKYLPCFQ VLPLDQQLVL VRNCWASLLM LELAQDRLQF ETVEVSEPSM LQKILTTRRR ETGGNEPLPV PTLQHHLAPP AEARKVPSAS QVQAIKCFLS KCWSLNISTK , corresponding to amino acids 96 - 376 of Human NR0B1/ Dax1 (NP_000466).
- WB: 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates IF: HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
Our Abpromise guarantee covers the use of ab97369 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionOrphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
Involvement in diseaseDefects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR0 subfamily.
DomainHomodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.
Cellular localizationNucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.
- Information by UniProt
- AHC antibody
- AHCH antibody
- AHX antibody
ab97369 has been referenced in 1 publication.
- Johnson KM et al. Role for the EWS domain of EWS/FLI in binding GGAA-microsatellites required for Ewing sarcoma anchorage independent growth. Proc Natl Acad Sci U S A 114:9870-9875 (2017). PubMed: 28847958