Immunocytochemistry/ Immunofluorescence abreview for Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Stem Cells)
Permeabilization
Yes - 0.2% Tritonx
Specification
Embryonic Stem Cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TritonX + 5% Donkey Serum in PBS
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.

Simon Hastreiter

Verified customer

Submitted Aug 15 2016

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