Overview

  • Product name
  • Description
    Rabbit polyclonal to NR0B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein corresponding to Human NR0B2.
    Database link: Q15466

  • Positive control
    • WB: Mouse liver and kidney tissue lysates. IHC-P: Rat liver, rat lung, rat brain and mouse brain tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab186874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 28 kDa.
ICC/IF 1/10 - 1/100.

Target

  • Function
    Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
  • Tissue specificity
    Liver. Low levels of expression were detected in heart and pancreas.
  • Involvement in disease
    Obesity
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR0 subfamily.
  • Post-translational
    modifications
    Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
  • Cellular localization
    Nucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR0B2 antibody
    • NR0B2_HUMAN antibody
    • Nr0b2a antibody
    • Nuclear receptor subfamily 0 group B member 2 antibody
    • Nuclear receptor subfamily 0, group B, member 2a antibody
    • Orphan nuclear receptor SHP antibody
    • SHP antibody
    • SHP-1 antibody
    • Shp1 antibody
    • Small heterodimer partner antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling NR0B2 with ab186874 at 2.38 mg/ml.

  • All lanes : Anti-NR0B2 antibody (ab186874) at 1/500 dilution

    Lane 1 : Mouse liver tissue extract
    Lane 2 : Mouse kidney tissue extract

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling NR0B2 with ab186874 at 1/100. Magnification: 400x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling NR0B2 with ab186874 at 1/100. Magnification: 400x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling NR0B2 with ab186874 at 1/100. Magnification: 400x.

References

This product has been referenced in:
  • Shahoei SH  et al. Small Heterodimer Partner Regulates Dichotomous T Cell Expansion by Macrophages. Endocrinology 160:1573-1589 (2019). Read more (PubMed: 31050726) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Liver)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 29 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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