Anti-NR0B2 antibody (ab232841)
Key features and details
- Rabbit polyclonal to NR0B2
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NR0B2 antibody
See all NR0B2 primary antibodies -
Description
Rabbit polyclonal to NR0B2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human NR0B2 aa 1 to the C-terminus. Two N-terminal tags. (Expressed in E.coli).
Database link: Q15466 -
Positive control
- IHC-P: Human liver tissue. WB: Recombinant human NR0B2 protein; Pig kidney lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
Target
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Function
Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. -
Tissue specificity
Liver. Low levels of expression were detected in heart and pancreas. -
Involvement in disease
Obesity -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR0 subfamily. -
Post-translational
modificationsArginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors. -
Cellular localization
Nucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8431 Human
- Entrez Gene: 100127458 Pig
- Omim: 604630 Human
- SwissProt: Q15466 Human
- Unigene: 427055 Human
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Alternative names
- NR0B2 antibody
- NR0B2_HUMAN antibody
- Nr0b2a antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232841 has not yet been referenced specifically in any publications.