Recombinant Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)
![Western blot - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-480013-AntiNR1D1antibodywb.jpg "Western blot - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10376] to NR1D1 + RVR
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NR1D1 + RVR antibody [EPR10376] -
Description
Rabbit monoclonal [EPR10376] to NR1D1 + RVR -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Y79, Hela, HepG2 and Jurkat cell lysates; Colon carcinoma tissue; Uterus tissue; Hela cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10376 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| IHC-P | (2) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 67 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. |
|
ICC/IF
1/100 - 1/250. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 67 kDa. |
Target
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Cellular localization
NR1D1: Nucleus. RVR: Nucleus. -
Database links
- Entrez Gene: 9572 Human
- Entrez Gene: 9975 Human
- Entrez Gene: 217166 Mouse
- Entrez Gene: 353187 Mouse
- Entrez Gene: 252917 Rat
- Entrez Gene: 259241 Rat
- Omim: 602304 Human
- Omim: 602408 Human
see all
Images
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Western blot - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)All lanes : Anti-NR1D1 + RVR antibody [EPR10376] (ab174309) at 1/1000 dilution
Lane 1 : GST-tagged human full length recombinant NR1D1 protein with NFDM/TBST
Lane 2 : GST-tagged human full length recombinant NR1D2 protein with NFDM/TBST
Lysates/proteins at 0.015 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 67 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 100 seconds -
![Western blot - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-199200-anti-nr1d1-antibody-epr10376-western-blot.jpg)
Western blot - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)All lanes : Anti-NR1D1 + RVR antibody [EPR10376] (ab174309) at 1/1000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 67 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-199204-anti-nr1d1-antibody-epr10376-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling NR1D1 with ab174309 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-199201-anti-nr1d1-antibody-epr10376-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)Immunofluorescent analysis of HeLa cells labeling NR1D1 with ab174309 at 1/100 dilution.
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![Flow Cytometry (Intracellular) - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-269707-anti-nr1d1-antibody-epr10376-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NR1D1 with ab174309 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-199202-anti-nr1d1-antibody-epr10376-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling NR1D1 with ab174309 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)](https://www.abcam.com/ps/products/174/ab174309/Images/ab174309-6-benefits-of-recombinant-antibodies.png)
Anti-NR1D1 + RVR antibody [EPR10376] (ab174309)
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab174309 has been referenced in 14 publications.
- Tian Y et al. Melatonin inhibits RANKL-induced osteoclastogenesis through the miR-882/Rev-erba axis in Raw264.7 cells. Int J Mol Med 47:633-642 (2021). PubMed: 33416111
- Griepentrog JE et al. Frontline Science: Rev-Erba links blue light with enhanced bacterial clearance and improved survival in murine Klebsiella pneumoniae pneumonia. J Leukoc Biol N/A:N/A (2019). PubMed: 31379019
- Tao L et al. Rev-erba inhibits proliferation by reducing glycolytic flux and pentose phosphate pathway in human gastric cancer cells. Oncogenesis 8:57 (2019). PubMed: 31591390
- Dierickx P et al. SR9009 has REV-ERB-independent effects on cell proliferation and metabolism. Proc Natl Acad Sci U S A 116:12147-12152 (2019). PubMed: 31127047
- Ji G et al. Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBa/ß pathway. Free Radic Biol Med 145:312-320 (2019). PubMed: 31585206
