Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris HCL ph10
Sample
Human Tissue sections (Cartilage)
Specification
Cartilage
Permeabilization
No
Fixative
zinc buffered formalin
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Vector's Goat Anti-Rabbit - Biotinylated
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin
Dilution
1/200
Additional data
Additional Notes
I used Vector's ABC kit with Alkaline Phosphatase and Red Substrate.
Abcam user community
Verified customer
Submitted Aug 01 2014