Application
Western blot
Sample
Human Tissue lysate - whole (ES cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
ES cells
Blocking step
5%BSA/1%Ovalbumin/0.05%Na Azide as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:5 block from above with TBS
Dilution
1/1000
Detection
Detection method
ECL Prime
Exposure
1 second(s)
Bands
Specific: 67 kDa Non-specific: >60 and >30 kDa
Positive control
I used ab29889 human liver tissue as a control. I have not included it in the image as there was a bubble obscuring the band (only end of band visible and wasn't very pretty). However, there was only a single band in the control running at the same size as the upper band in the human ES cell sample shown in blot.
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Submitted Apr 04 2016