Recombinant
RabMAb

Recombinant Anti-NR1D1 antibody [EPR10376] - BSA and Azide free (ab236008)

Overview

  • Product name

    Anti-NR1D1 antibody [EPR10376] - BSA and Azide free
    See all NR1D1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10376] to NR1D1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NR1D1 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P20393

  • Positive control

    • IHC-P: Human uterus tissue.
  • General notes

    Ab236008 is the carrier-free version of ab174309. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236008 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 67 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.
  • Tissue specificity

    Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAR-1 antibody
    • EAR1 antibody
    • ERBA-related 1 antibody
    • hRev antibody
    • Nr1d1 antibody
    • NR1D1_HUMAN antibody
    • Nuclear receptor Rev ErbA alpha antibody
    • Nuclear receptor subfamily 1 group D member 1 antibody
    • Rev erbAalpha antibody
    • Rev erbalpha antibody
    • Rev-erbA-alpha antibody
    • Rev-ErbAalpha antibody
    • Reverba antibody
    • THRA1 antibody
    • THRAL antibody
    • Thyroid hormone receptor, alpha like antibody
    • Thyroid hormone receptor, alpha-1- like antibody
    • V-erbA-related protein 1 antibody
    see all

Images

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NR1D1 with ab174309 at 1/20 dilution(10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling NR1D1 with ab174309 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

  • Immunofluorescent analysis of HeLa cells labeling NR1D1 with ab174309 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling NR1D1 with ab174309 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174309).

References

ab236008 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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