Recombinant
RabMAb

Recombinant Anti-NR1D1 antibody [EPR10376] (FITC) (ab225245)

Overview

  • Product name

    Anti-NR1D1 antibody [EPR10376] (FITC)
    See all NR1D1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10376] to NR1D1 (FITC)
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human NR1D1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P20393

  • Positive control

    • Flow cyt: HeLa cells. ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

 

ICC/IF 1/5000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.
  • Tissue specificity

    Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAR-1 antibody
    • EAR1 antibody
    • ERBA-related 1 antibody
    • hRev antibody
    • Nr1d1 antibody
    • NR1D1_HUMAN antibody
    • Nuclear receptor Rev ErbA alpha antibody
    • Nuclear receptor subfamily 1 group D member 1 antibody
    • Rev erbAalpha antibody
    • Rev erbalpha antibody
    • Rev-erbA-alpha antibody
    • Rev-ErbAalpha antibody
    • Reverba antibody
    • THRA1 antibody
    • THRAL antibody
    • Thyroid hormone receptor, alpha like antibody
    • Thyroid hormone receptor, alpha-1- like antibody
    • V-erbA-related protein 1 antibody
    see all

Images

  • ab225245 staining NR1D1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225245 at 1/5000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab225245 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225245, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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