Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18346-44] to NR1D1 (phospho S55 + S59)
- Suitable for: WB, ELISA
- Reacts with: Human
Product nameAnti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44]
See all NR1D1 primary antibodies
DescriptionRabbit monoclonal [EPR18346-44] to NR1D1 (phospho S55 + S59)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NR1D1 aa 50-150 (phospho S55 + S59). The exact sequence is proprietary.
Database link: P20393
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 67 kDa).|
|ELISA||Use at an assay dependent concentration.|
FunctionFunctions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.
Tissue specificityExpressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- EAR-1 antibody
- EAR1 antibody
- ERBA-related 1 antibody
Direct ELISA analysis of ab191218 (0-1000 ng/ml) using the following antigens: NR1D1 phospho S55 + phospho S59 peptide, NR1D1 phospho S55 peptide, NR1D1 phospho S59 peptide and NR1D1 unmodified peptide at 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 was used as secondary antibody.
ab191218 has not yet been referenced specifically in any publications.