RecombinantRabMAb

Recombinant Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)

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Western blot - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)
  • ELISA - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)
  • Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18346-44] to NR1D1 (phospho S55 + S59)
  • Suitable for: WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44]
    See all NR1D1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18346-44] to NR1D1 (phospho S55 + S59)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human NR1D1 aa 50-150 (phospho S55 + S59). The exact sequence is proprietary.
    Database link: P20393

  • Positive control

    • WB: 293T transfected with NR1D1 expression vector containing a GFP-tag®, whole cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab191218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 67 kDa).
ELISA Use at an assay dependent concentration.

Target

  • Function

    Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.

  • Tissue specificity

    Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • EAR-1 antibody
      • EAR1 antibody
      • ERBA-related 1 antibody
      • hRev antibody
      • Nr1d1 antibody
      • NR1D1_HUMAN antibody
      • Nuclear receptor Rev ErbA alpha antibody
      • Nuclear receptor subfamily 1 group D member 1 antibody
      • Rev erbAalpha antibody
      • Rev erbalpha antibody
      • Rev-erbA-alpha antibody
      • Rev-ErbAalpha antibody
      • Reverba antibody
      • THRA1 antibody
      • THRAL antibody
      • Thyroid hormone receptor, alpha like antibody
      • Thyroid hormone receptor, alpha-1- like antibody
      • V-erbA-related protein 1 antibody
      see all

    Images

    • Western blot - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)
      Western blot - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)
      All lanes : Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218) at 1/5000 dilution (Purified)

      Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a GFP-tag®, whole cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with NR1D1 expression vector containing a GFP-tag®, whole cell lysate
      Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with NR1D1 expression vector containing a GFP-tag® whole cell lysate,then the membrane treated with Phosphatase for 1 hour

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 67 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    • ELISA - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)
      ELISA - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)

      Direct ELISA analysis of ab191218 (0-1000 ng/ml) using the following antigens: NR1D1 phospho S55 + phospho S59 peptide, NR1D1 phospho S55 peptide, NR1D1 phospho S59 peptide and NR1D1 unmodified peptide at 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 was used as secondary antibody. 

    • Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)
      Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218)

    References (0)

    Publishing research using ab191218? Please let us know so that we can cite the reference in this datasheet.

    ab191218 has not yet been referenced specifically in any publications.

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