Overview

  • Product name
    Anti-NR2F2 antibody [H7147]
    See all NR2F2 primary antibodies
  • Description
    Mouse monoclonal [H7147] to NR2F2
  • Host species
    Mouse
  • Specificity
    This antibody does not recognize human COUP TF1 and EAR2.
  • Tested applications
    Suitable for: ELISA, IHC-P, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment:

    PGQGGPAS TPAQTAAGGQ GGPG

    , corresponding to amino acids 43-64 of Human NR2F2

  • Positive control
    • Human Convoluted tubule and Rat Glomerular

Properties

Applications

Our Abpromise guarantee covers the use of ab41859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).

Target

  • Function
    Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Congenital heart defects, multiple types, 4
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apolipoprotein A-I regulatory protein 1 antibody
    • Apolipoprotein AI regulatory protein 1 antibody
    • ARP-1 antibody
    • ARP1 antibody
    • COT2_HUMAN antibody
    • COUP TF2 antibody
    • COUP transcription factor 2 antibody
    • COUP transcription factor II antibody
    • COUP-TF II antibody
    • COUP-TF2 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 2 antibody
    see all

Images

  • Immnocytochemical immunoflurescence analysis of PFA-fixed human pluripotent stem cell derived cardiomyocytes, labelling NR2F2 with ab41859 at a concentration of 1/1000 incubated for 16 hours at 4°C. Permeablisation is using saponin. Blocking was done with 5% serum for 15 minutes at room temperature. Secondary used was a goat anti-mouse Alexa Fluor® 568 at 1/2000. The left panel shows the experimental conditions described formerly. The right panel is additionally counterstained with DAPI to show all cell nuclei within the area.

    See Abreview

  • ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
  • ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
  • All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References

This product has been referenced in:
  • Argenziano M  et al. Electrophysiologic Characterization of Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Atrial Cardiomyocytes. Stem Cell Reports 10:1867-1878 (2018). Read more (PubMed: 29731429) »
  • Lilis I  et al. The expression of p-mTOR and COUP-TFII correlates with increased lymphangiogenesis and lymph node metastasis in prostate adenocarcinoma. Urol Oncol 36:311.e27-311.e35 (2018). Read more (PubMed: 29544697) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Mouse Cell (BRAIN)
Permeabilization
Yes - 4% paraform
Specification
BRAIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Kyungjoo Seong

Verified customer

Submitted Jun 20 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human pluripotent stem cell derived cardiomyocytes)
Permeabilization
Yes - saponin
Specification
human pluripotent stem cell derived cardiomyocytes
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 20 2015

Answer

Thank you for your reply.
xxxx is currently away from the office but I will try to help with your request.
xxxx
I am very pleased to hear you would like to accept our offer and test ab41859 in ChIP. This code will give you €370 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:
www.abcam.com/Abreviews
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here:
www.abcam.com/abtrial

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Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab41859 NR2F2 antibody [H7147] has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Prices are provided on the individual datasheets. Select the correct country from the dropdown menu at the top right corner of the page, and the correct price and shipping costs for that country will show at the top of the datasheet.

ab41859 Price for Italy: 100 µl €370.00
Shipping: €29.00 to Italy

If you would like to test ab41859 in ChIP, please do not hesitate to contact me again prior to the purchase by replying to this message as you may be eligible for our AbTrial testing program. Please see the following page from our website for more information:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&source=pagetrap&viapagetrap=abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:
https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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