Product nameAnti-NR2F2 antibody [H7147]
See all NR2F2 primary antibodies
DescriptionMouse monoclonal [H7147] to NR2F2
SpecificityThis antibody does not recognize human COUP TF1 and EAR2.
Tested applicationsSuitable for: ELISA, IHC-P, IP, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- Human Convoluted tubule and Rat Glomerular
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Purification notesPurified from ascites by ammonium sulfate fractionation.
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab41859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.1 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).|
FunctionLigand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.
Involvement in diseaseCongenital heart defects, multiple types, 4
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- Apolipoprotein A-I regulatory protein 1 antibody
- Apolipoprotein AI regulatory protein 1 antibody
- ARP-1 antibody
Immnocytochemical immunoflurescence analysis of PFA-fixed human pluripotent stem cell derived cardiomyocytes, labelling NR2F2 with ab41859 at a concentration of 1/1000 incubated for 16 hours at 4°C. Permeablisation is using saponin. Blocking was done with 5% serum for 15 minutes at room temperature. Secondary used was a goat anti-mouse Alexa Fluor® 568 at 1/2000. The left panel shows the experimental conditions described formerly. The right panel is additionally counterstained with DAPI to show all cell nuclei within the area.
ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This product has been referenced in:
- Argenziano M et al. Electrophysiologic Characterization of Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Atrial Cardiomyocytes. Stem Cell Reports 10:1867-1878 (2018). Read more (PubMed: 29731429) »
- Lilis I et al. The expression of p-mTOR and COUP-TFII correlates with increased lymphangiogenesis and lymph node metastasis in prostate adenocarcinoma. Urol Oncol 36:311.e27-311.e35 (2018). Read more (PubMed: 29544697) »