Product nameAnti-NR2F6 / EAR-2 antibody
See all NR2F6 / EAR-2 primary antibodies
DescriptionRabbit polyclonal to NR2F6 / EAR-2
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- HepG2 cell lysate
Protein previously labeled as NR2F6.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab49222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionTranscription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
Tissue specificityExpressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- EAR 2 antibody
- EAR-2 antibody
- EAR2 antibody
Anti-NR2F6 / EAR-2 antibody (ab49222) at 5 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
ab49222 has not yet been referenced specifically in any publications.