Anti-NR2F6/EAR-2 antibody (ab65012)
Key features and details
- Rabbit polyclonal to NR2F6/EAR-2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NR2F6/EAR-2 antibody
See all NR2F6/EAR-2 primary antibodies -
Description
Rabbit polyclonal to NR2F6/EAR-2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/1000.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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Notes |
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ICC/IF
1/500 - 1/1000. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). -
Tissue specificity
Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2063 Human
- Omim: 132880 Human
- SwissProt: P10588 Human
- Unigene: 466148 Human
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Alternative names
- EAR 2 antibody
- EAR-2 antibody
- EAR2 antibody
see all
Images
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All lanes : Anti-NR2F6/EAR-2 antibody (ab65012) at 1/500 dilution
Lane 1 : HeLa cell extract at 10-30µg
Lane 2 : 293 cell extract at 10-30µg
Lane 3 : HUVEC cell extract at 10-30µg
Lane 4 : HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg
Predicted band size: 43 kDa
Observed band size: 43 kDa -
ab65012 at 1/500 - 1/1000 dilution staining NR2F6/EAR-2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6/EAR-2 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab65012 has been referenced in 2 publications.
- Weatherford ET et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol 302:F1025-33 (2012). WB . PubMed: 22278040
- Modica S et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P ; Human . PubMed: 19818784