Anti-NR2F6/EAR-2 antibody (ab137496)
Key features and details
- Rabbit polyclonal to NR2F6/EAR-2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NR2F6/EAR-2 antibody
See all NR2F6/EAR-2 primary antibodies -
Description
Rabbit polyclonal to NR2F6/EAR-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human NR2F6/EAR-2 aa 344-404. The exact sequence is proprietary.
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Positive control
- HeLa, Raji, NIH 3T3 and JC whole cell lysates, Human gastric carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH8
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH8 |
Target
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Function
Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). -
Tissue specificity
Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2063 Human
- Entrez Gene: 13864 Mouse
- Omim: 132880 Human
- SwissProt: P10588 Human
- SwissProt: P43136 Mouse
- Unigene: 466148 Human
- Unigene: 28989 Mouse
- Unigene: 440170 Mouse
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Alternative names
- EAR 2 antibody
- EAR-2 antibody
- EAR2 antibody
see all
Images
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All lanes : Anti-NR2F6/EAR-2 antibody (ab137496) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS PAGE -
All lanes : Anti-NR2F6/EAR-2 antibody (ab137496) at 1/500 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling NR2F6/EAR-2 with ab137496 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137496 has been referenced in 4 publications.
- Zhou B et al. The Nuclear Orphan Receptor NR2F6 Promotes Hepatic Steatosis through Upregulation of Fatty Acid Transporter CD36. Adv Sci (Weinh) 7:2002273 (2020). PubMed: 33173745
- Klapper L et al. NR2F6 as a Prognostic Biomarker in HNSCC. Int J Mol Sci 21:N/A (2020). PubMed: 32752295
- Wang L et al. Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression. Mol Cancer 18:119 (2019). PubMed: 31324186
- Klepsch V et al. Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade. Nat Commun 9:1538 (2018). PubMed: 29670099