Overview

  • Product name

  • Description

    Rabbit polyclonal to NR2F6/EAR-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human NR2F6/EAR-2 aa 344-404. The exact sequence is proprietary.

  • Positive control

    • HeLa, Raji, NIH 3T3 and JC whole cell lysates, Human gastric carcinoma tissue.
  • General notes

    Protein previously labeled as NR2F6.

Properties

Applications

Our Abpromise guarantee covers the use of ab137496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH8

Target

  • Function

    Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
  • Tissue specificity

    Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAR 2 antibody
    • EAR-2 antibody
    • EAR2 antibody
    • ERBA RELATED 2 antibody
    • ERBA related gene 2 antibody
    • ERBAL2 antibody
    • Nr2f6 antibody
    • NR2F6_HUMAN antibody
    • Nuclear receptor subfamily 2 group F member 6 antibody
    • Orphan nuclear receptor EAR2 (V erbA related protein EAR 2) antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like antibody
    • V erbA related protein EAR 2 antibody
    • V-erbA-related protein 2 antibody
    see all

Images

  • All lanes : Anti-NR2F6/EAR-2 antibody (ab137496) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 43 kDa



    10% SDS PAGE
  • All lanes : Anti-NR2F6/EAR-2 antibody (ab137496) at 1/500 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling NR2F6/EAR-2 with ab137496 at 1/100 dilution.

References

This product has been referenced in:

  • Wang L  et al. Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression. Mol Cancer 18:119 (2019). Read more (PubMed: 31324186) »
  • Klepsch V  et al. Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade. Nat Commun 9:1538 (2018). Read more (PubMed: 29670099) »
See all 2 Publications for this product

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