Key features and details
- Rabbit polyclonal to NR2F6/EAR-2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NR2F6/EAR-2 antibody
See all NR2F6/EAR-2 primary antibodies
DescriptionRabbit polyclonal to NR2F6/EAR-2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human NR2F6/EAR-2 (N terminal).
Protein previously labeled as NR2F6.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
FunctionTranscription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
Tissue specificityExpressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- EAR 2 antibody
- EAR-2 antibody
- EAR2 antibody
All lanes : Anti-NR2F6/EAR-2 antibody (ab65012) at 1/500 dilution
Lane 1 : HeLa cell extract at 10-30µg
Lane 2 : 293 cell extract at 10-30µg
Lane 3 : HUVEC cell extract at 10-30µg
Lane 4 : HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg
Predicted band size: 43 kDa
Observed band size: 43 kDa
ab65012 at 1/500 - 1/1000 dilution staining NR2F6/EAR-2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6/EAR-2 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
ab65012 has been referenced in 2 publications.
- Weatherford ET et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol 302:F1025-33 (2012). WB . PubMed: 22278040
- Modica S et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P ; Human . PubMed: 19818784