Anti-NR5A2 / LRH1 antibody (ab219603)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NR5A2 / LRH1 antibody
See all NR5A2 / LRH1 primary antibodies -
Description
Rabbit polyclonal to NR5A2 / LRH1 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Gorilla
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Immunogen
Synthetic peptide corresponding to Human NR5A2/ LRH1. 16 amino acid peptide from ligand-binding domain.
Database link: O00482 -
Positive control
- Human liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.099% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab219603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 - 19 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. -
Tissue specificity
Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395961 Chicken
- Entrez Gene: 469631 Chimpanzee
- Entrez Gene: 541305 Cow
- Entrez Gene: 490252 Dog
- Entrez Gene: 101142071 Gorilla
- Entrez Gene: 100009700 Horse
- Entrez Gene: 2494 Human
- Entrez Gene: 26424 Mouse
see all -
Alternative names
- Alpha-1-fetoprotein transcription factor antibody
- B1-binding factor antibody
- B1F antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A2 / LRH1 antibody (ab219603)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human liver tissue labeling NR5A2 / LRH1 with ab219603 at 19μg/ml dilution.
Protocols
Datasheets and documents
References
ab219603 has not yet been referenced specifically in any publications.
