Anti-NR5A2 / LRH1 antibody (ab153944)
Key features and details
- Rabbit polyclonal to NR5A2 / LRH1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NR5A2 / LRH1 antibody
See all NR5A2 / LRH1 primary antibodies -
Description
Rabbit polyclonal to NR5A2 / LRH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 143-541 of Human NR5A2/ LRH1 (Uniprot ID O00482).
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Positive control
- Recombinant Human NR5A2 / LRH1 protein (ab81844) can be used as a positive control in WB. Mouse liver whole cell lysate; Human ESC cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. -
Tissue specificity
Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395961 Chicken
- Entrez Gene: 541305 Cow
- Entrez Gene: 2494 Human
- Entrez Gene: 26424 Mouse
- Entrez Gene: 60349 Rat
- Entrez Gene: 496513 Xenopus tropicalis
- Omim: 604453 Human
- SwissProt: O42101 Chicken
see all -
Alternative names
- Alpha-1-fetoprotein transcription factor antibody
- B1-binding factor antibody
- B1F antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab153944 has been referenced in 4 publications.
- Song Y et al. LRH1 Acts as an Oncogenic Driver in Human Osteosarcoma and Pan-Cancer. Front Cell Dev Biol 9:643522 (2021). PubMed: 33791301
- van Veen JE et al. Mutationally-activated PI3'-kinase-a promotes de-differentiation of lung tumors initiated by the BRAFV600E oncoprotein kinase. Elife 8:N/A (2019). PubMed: 31452510
- Liu Y et al. LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non-small cell lung cancer. Thorac Cancer 9:1725-1732 (2018). PubMed: 30273983
- Guo J et al. Liver receptor homolog 1 influences blastocyst hatching in pigs. J Reprod Dev 62:297-303 (2016). IF . PubMed: 26971889