Overview

  • Product name

    Anti-NR5A2 / LRH1 antibody
    See all NR5A2 / LRH1 primary antibodies
  • Description

    Rabbit polyclonal to NR5A2 / LRH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 143-541 of Human NR5A2/ LRH1 (Uniprot ID O00482).

  • Positive control

    • Recombinant Human NR5A2 / LRH1 protein (ab81844) can be used as a positive control in WB. Mouse liver whole cell lysate; Human ESC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
  • Tissue specificity

    Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-1-fetoprotein transcription factor antibody
    • B1-binding factor antibody
    • B1F antibody
    • B1F2 antibody
    • CPF antibody
    • CYP7A promoter-binding factor antibody
    • FTF antibody
    • FTZ F1 antibody
    • FTZ F1beta antibody
    • FTZ-F1 antibody
    • FTZ-F1beta antibody
    • hB1F 2 antibody
    • hB1F antibody
    • hB1F-2 antibody
    • Hepatocytic transcription factor antibody
    • Liver receptor homolog 1 antibody
    • LRH-1 antibody
    • LRH1 antibody
    • Nr5a2 antibody
    • NR5A2_HUMAN antibody
    • Nuclear receptor subfamily 5 group A member 2 antibody
    see all

Images

  • Anti-NR5A2 / LRH1 antibody (ab153944) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, labeling NR5A2 / LRH1 with ab153944 at 1/400 dilution. Blue: Hoechst 33342 staining.

References

This product has been referenced in:

  • Liu Y  et al. LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non-small cell lung cancer. Thorac Cancer 9:1725-1732 (2018). Read more (PubMed: 30273983) »
  • Guo J  et al. Liver receptor homolog 1 influences blastocyst hatching in pigs. J Reprod Dev 62:297-303 (2016). IF . Read more (PubMed: 26971889) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Cow Cell lysate - whole cell (Luteal cells)
Gel Running Conditions
Reduced Denaturing (2% SDS)
Loading amount
30 µg
Specification
Luteal cells
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 06 2018

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