Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876)
Key features and details
- Rabbit polyclonal to NR5A2 / LRH1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NR5A2 / LRH1 antibody - C-terminal
See all NR5A2 / LRH1 primary antibodies -
Description
Rabbit polyclonal to NR5A2 / LRH1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human NR5A2/ LRH1 aa 250 to the C-terminus (C terminal).
Database link: O00482 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 61 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 61 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. -
Tissue specificity
Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2494 Human
- Entrez Gene: 26424 Mouse
- Entrez Gene: 60349 Rat
- Omim: 604453 Human
- SwissProt: O00482 Human
- SwissProt: P45448 Mouse
- SwissProt: Q9QWM1 Rat
- Unigene: 33446 Human
see all -
Alternative names
- Alpha-1-fetoprotein transcription factor antibody
- B1-binding factor antibody
- B1F antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab189876. Blue DAPI for nuclear staining.
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All lanes : Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876) at 1/500 dilution
Lane 1 : MCF7 cell line extract
Lane 2 : 293T cell line extract
Lane 3 : HeLa cell line extract
Lane 4 : Mouse liver extract
Lane 5 : Rat liver extract
Predicted band size: 61 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab189876 has been referenced in 8 publications.
- Boftsi M et al. The adeno-associated virus 2 genome and Rep 68/78 proteins interact with cellular sites of DNA damage. Hum Mol Genet 31:985-998 (2022). PubMed: 34652429
- Song Y et al. LRH1 Acts as an Oncogenic Driver in Human Osteosarcoma and Pan-Cancer. Front Cell Dev Biol 9:643522 (2021). PubMed: 33791301
- Tan X et al. Lgr4 Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway. Front Cell Dev Biol 9:666303 (2021). PubMed: 34631693
- Yang Q et al. NR5A2 Promotes Cell Growth and Resistance to Temozolomide Through Regulating Notch Signal Pathway in Glioma. Onco Targets Ther 13:10231-10244 (2020). PubMed: 33116604
- Majumder K et al. The NS1 protein of the parvovirus MVM Aids in the localization of the viral genome to cellular sites of DNA damage. PLoS Pathog 16:e1009002 (2020). PubMed: 33064772
- Sladitschek HL & Neveu PA A gene regulatory network controls the balance between mesendoderm and ectoderm at pluripotency exit. Mol Syst Biol 15:e9043 (2019). PubMed: 31885203
- Majumder K et al. Parvovirus minute virus of mice interacts with sites of cellular DNA damage to establish and amplify its lytic infection. Elife 7:N/A (2018). PubMed: 30028293
- Liu Y et al. LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non-small cell lung cancer. Thorac Cancer 9:1725-1732 (2018). PubMed: 30273983