Key features and details
- Rabbit polyclonal to NR5A2 / LRH1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NR5A2 / LRH1 antibody - C-terminal
See all NR5A2 / LRH1 primary antibodies
DescriptionRabbit polyclonal to NR5A2 / LRH1 - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human NR5A2/ LRH1 aa 252-541 (C terminal).
HGSLQGYQTYGHFPSRAIKSEYPDPYTSSPESIMGYSYMDSYQTSSPASI PHLILELLKCEPDEPQVQAKIMAYLQQEQANRSKHEKLSTFGLMCKMADQ TLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVVHGKEG SIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCL KFLVLFSLDVKNLENFQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLL RLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEMLHAKRA
Database link: O00482
- MCF7, 293T, HeLa, Mouse liver and Rat liver extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 61 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionBinds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
Tissue specificityAbundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- Alpha-1-fetoprotein transcription factor antibody
- B1-binding factor antibody
- B1F antibody
Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab189876. Blue DAPI for nuclear staining.
All lanes : Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876) at 1/500 dilution
Lane 1 : MCF7 cell line extract
Lane 2 : 293T cell line extract
Lane 3 : HeLa cell line extract
Lane 4 : Mouse liver extract
Lane 5 : Rat liver extract
Predicted band size: 61 kDa
ab189876 has been referenced in 2 publications.
- Sladitschek HL & Neveu PA A gene regulatory network controls the balance between mesendoderm and ectoderm at pluripotency exit. Mol Syst Biol 15:e9043 (2019). PubMed: 31885203
- Liu Y et al. LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non-small cell lung cancer. Thorac Cancer 9:1725-1732 (2018). PubMed: 30273983