Overview

  • Product name

  • Description

    Rabbit polyclonal to NRAMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NRAMP1 aa 256-285 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P49279

  • Positive control

    • HepG2 cells; CEM cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab175986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 60 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function

    Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
  • Tissue specificity

    Macrophages; peripheral blood leukocytes, lung, spleen and liver.
  • Sequence similarities

    Belongs to the NRAMP family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • LSH antibody
    • Natural resistance associated macrophage protein 1 antibody
    • Natural resistance-associated macrophage protein 1 antibody
    • NRAM1_HUMAN antibody
    • NRAMP 1 antibody
    • NRAMP antibody
    • PBC antibody
    • SLC11A1 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1 antibody
    • solute carrier family 11 (sodium/phosphate symporters) member 1 antibody
    • Solute carrier family 11 member 1 antibody
    see all

Images

  • Anti-NRAMP1 antibody (ab175986) at 1/1000 dilution + CEM cell line lysates at 35 µg

    Predicted band size: 60 kDa

  • Confocal immunofluorescent analysis of HepG2 cells labeling NRAMP1 with ab175986 at 1/10 followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

References

ab175986 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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