Key features and details
- Rabbit polyclonal to NRAP
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NRAP antibody
DescriptionRabbit polyclonal to NRAP
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
FunctionMay be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
Tissue specificityExpressed in cardiac and skeletal muscle.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 44 nebulin repeats.
Cellular localizationLocalized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle.
- Information by UniProt
- N-RAP antibody
- Nebulin-related-anchoring protein antibody
- Nrap antibody
- NRAP_HUMAN antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.
ab122427 has been referenced in 2 publications.
- Li F et al. Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function. Skelet Muscle 10:2 (2020). PubMed: 31992366
- Truszkowska GT et al. Homozygous truncating mutation in NRAP gene identified by whole exome sequencing in a patient with dilated cardiomyopathy. Sci Rep 7:3362 (2017). WB ; Human . PubMed: 28611399