Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 5.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||Use at an assay dependent concentration. Predicted molecular weight: 197 kDa.|
FunctionMay be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
Tissue specificityExpressed in cardiac and skeletal muscle.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 44 nebulin repeats.
Cellular localizationLocalized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle.
- Information by UniProt
- N-RAP antibody
- Nebulin-related-anchoring protein antibody
- Nrap antibody
- NRAP_HUMAN antibody
Anti-NRAP antibody (ab122427) at 1/1000 dilution + Sheep cardiac tissue at 10 µg
Donkey Anti-Rabbit IgG (H+L) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 197 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 50 seconds
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.