Anti-NRAS antibody - C-terminal (ab167136)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-NRAS antibody - C-terminal
See all NRAS primary antibodies -
DescriptionRabbit polyclonal to NRAS - C-terminal
-
Host speciesRabbit
-
Specificityab167136 is predicted to not cross-react with H-RAS.
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide, corresponding to 15 amino acids from near the C terminus of Human NRAS (NP_002515).
-
Positive control
- 293 cell lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab167136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
Target
-
FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
-
Involvement in diseaseDefects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits. -
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
-
Post-translational
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. -
Cellular localizationCell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
- Information by UniProt
-
Database links
- Entrez Gene: 4893 Human
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24605 Rat
- Omim: 164790 Human
- SwissProt: P01111 Human
- SwissProt: P08556 Mouse
- SwissProt: Q04970 Rat
- Unigene: 486502 Human
see all -
Alternative names
- ALPS4 antibody
- AV095280 antibody
- GTPase NRas antibody
see all
Images
-
Western blot - Anti-NRAS antibody - C-terminal (ab167136)Western Blot of A431 cell lysate labeling NRAS with Anti-NRAS antibody - C-terminal (ab167136) at (1) 1 and (2) 2µg/ml. -
Western blot - Anti-NRAS antibody - C-terminal (ab167136)Lane 1 : Anti-NRAS antibody - C-terminal (ab167136) at 1 µg/ml
Lane 2 : Anti-NRAS antibody - C-terminal (ab167136) at 2 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 21 kDa
Protocols
References
This product has been referenced in:
- Martinez-Useros J et al. UNR/CDSE1 expression as prognosis biomarker in resectable pancreatic ductal adenocarcinoma patients: A proof-of-concept. PLoS One 12:e0182044 (2017). Read more (PubMed: 28763470) »
