Overview

  • Product name

    Anti-NRAS (mutated Q61) antibody [EPR20278]
    See all NRAS primary antibodies
  • Description

    Rabbit monoclonal [EPR20278] to NRAS (mutated Q61)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NRAS aa 50-150 (mutated Q61). The exact sequence is proprietary. Q61R
    Database link: P01111

  • Positive control

    • WB: SK-MEL-2 and HT-1197 whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease

    Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Images

  • All lanes : Anti-NRAS (mutated Q61) antibody [EPR20278] (ab222516) at 1/1000 dilution

    Lane 1 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
    Lane 2 : HT-1197 (human urinary bladder carcinoma cell line) whole cell lysate
    Lane 3 : MeWo (human malignant melanoma fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution: 5% NFDM/TBST.

    SK-MEL-2 cell line contains N-Ras Q61R homozygous mutation. HT-1197 cell line contains N-Ras Q61R heterozygous mutation. MeWo cell line is wild-type for N-Ras.

     

     

     

References

ab222516 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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