Product nameAnti-NRAS (mutated Q61) antibody [EPR20278]
See all NRAS primary antibodies
DescriptionRabbit monoclonal [EPR20278] to NRAS (mutated Q61)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NRAS aa 50-150 (mutated Q61). The exact sequence is proprietary. Q61R
Database link: P01111
- WB: SK-MEL-2 and HT-1197 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).|
FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
Involvement in diseaseDefects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
Cellular localizationCell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
- Information by UniProt
- ALPS4 antibody
- AV095280 antibody
- GTPase NRas antibody
All lanes : Anti-NRAS (mutated Q61) antibody [EPR20278] (ab222516) at 1/1000 dilution
Lane 1 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
Lane 2 : HT-1197 (human urinary bladder carcinoma cell line) whole cell lysate
Lane 3 : MeWo (human malignant melanoma fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking/Dilution: 5% NFDM/TBST.
SK-MEL-2 cell line contains N-Ras Q61R homozygous mutation. HT-1197 cell line contains N-Ras Q61R heterozygous mutation. MeWo cell line is wild-type for N-Ras.
ab222516 has not yet been referenced specifically in any publications.