Overview

  • Product name

    Anti-NRAS (mutated Q61 R) antibody [SP174]
    See all NRAS primary antibodies
  • Description

    Rabbit monoclonal [SP174] to NRAS (mutated Q61 R)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NRAS aa 50-150 (mutated Q61 R). The exact sequence is proprietary.
    Database link: P01111

  • Positive control

    • WB: SK-MEL-2 cell lysate. IHC-P: SK-MEL-2 cells. FC: SK-MEL-2

Properties

Applications

Our Abpromise guarantee covers the use of ab227658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000.
WB 1/400. Predicted molecular weight: 21 kDa.
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Target

  • Function

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease

    Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • Cellular localization

    Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Images

  • Flow Cytometry analysis of SK-MEL-2( Human skin malignant melanoma) cells labeling NRAS with purifiedab227658 at 1:200 dilution (0.8 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Anti-NRAS (mutated Q61 R) antibody [SP174] (ab227658) at 1/400 dilution + SK-MEL-2 cell lysate

    Predicted band size: 21 kDa

  • Formalin-fixed, paraffin-embedded human SK-MEL-2 cells stained for NRAS (mutated Q61 R) using ab227658 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Pellegrini C  et al. Heterogeneity of BRAF, NRAS, and TERT Promoter Mutational Status in Multiple Melanomas and Association with MC1R Genotype: Findings from Molecular and Immunohistochemical Analysis. J Mol Diagn 20:110-122 (2018). Read more (PubMed: 29061376) »
  • Manevitz-Mendelson E  et al. Somatic NRAS mutation in patient with generalized lymphatic anomaly. Angiogenesis N/A:N/A (2018). Read more (PubMed: 29397482) »
See all 7 Publications for this product

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