Western blot abreview for Anti-NRAS (mutated Q61R) antibody [SP174]

Western blot

Human Cell lysate - whole cell (VMM39 cells and HeLa cells)

Reduced Denaturing (12%)

20 µg

NRAS siRNA (10 nM), 72 hrs

VMM39 cells and HeLa cells

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA in TBS-T

1/500

Non-Abcam antibody was used: HRP-conjugated affinipure goat anti-rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

ECL

15 second(s)

Specific: 22 kDa

Lane 1: VMM39 cells (NRAS Q61K), non-targeting control siRNA, 72 hrs Lane 2: VMM39 cells (NRAS Q61K), NRAS siRNA (10 nM), 72 hrs Lane 3: HeLa cells (NRAS WT) This antibody is is able to discriminate between NRAS Q61 mutants and NRAS WT. Although this antibody is marketed as an NRAS Q61R antibody, it also recognizes NRAS Q61K.