Key features and details
- Rabbit polyclonal to NRBF2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NRBF2 antibody
See all NRBF2 primary antibodies
DescriptionRabbit polyclonal to NRBF2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 237 and 287 of Human NRBF2 (NP_110386.2).
- Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 32 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).
Tissue specificityDetected in keratinocytes, liver and placenta.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- Comodulator of PPAR and RXR 1 antibody
- Comodulator of PPAR and RXR 2 antibody
- Comodulator of PPAR and RXR antibody
All lanes : Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from 293T cells at 50 µg
Lane 5 : Whole cell
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab85733 has not yet been referenced specifically in any publications.