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Rabbit polyclonal to NRBF2
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 237 and 287 of Human NRBF2 (NP_110386.2).
Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
Use at 2-5 µg/mg of lysate.
May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).
Detected in keratinocytes, liver and placenta.
Information by UniProt
Comodulator of PPAR and RXR 1 antibody
Comodulator of PPAR and RXR 2 antibody
Comodulator of PPAR and RXR antibody
Western blot - Anti-NRBF2 antibody (ab85733)
All lanes :
Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml
Lane 1 :
lysate from HeLa cells at 50 µg
Lane 2 :
lysate from HeLa cells at 15 µg
Lane 3 :
lysate from HeLa cells at 5 µg
Lane 4 :
lysate from 293T cells at 50 µg
Lane 5 :
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
40 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-NRBF2 antibody (ab85733)
Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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