Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15654] to NrCAM
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-NrCAM antibody [EPR15654]
See all NrCAM primary antibodies
DescriptionRabbit monoclonal [EPR15654] to NrCAM
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human NrCAM aa 800-950. The exact sequence is proprietary.
Database link: Q92823
- Human glioma and forebrain lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).|
FunctionCell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
Tissue specificityDetected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
Sequence similaritiesBelongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- Bravo antibody
- hBravo antibody
- Neuronal cell adhesion molecule antibody
Anti-NrCAM antibody [EPR15654] (ab191418) at 1/50000 dilution + Human glioma lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 144 kDa
Western blot analysis of immunoprecipitation pellet from Human glioma lysate immunoprecipitated using ab191418 at 1/30 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-NrCAM antibody [EPR15654] (ab191418) at 1/10000 dilution + Human forebrain lysate at 10 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 144 kDa
ab191418 has not yet been referenced specifically in any publications.