Overview

  • Product name

    Anti-NrCAM antibody [EPR15654]
    See all NrCAM primary antibodies
  • Description

    Rabbit monoclonal [EPR15654] to NrCAM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NrCAM aa 800-950. The exact sequence is proprietary.
    Database link: Q92823

  • Positive control

    • Human glioma and forebrain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15654
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/10000 - 1/50000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).

Target

  • Function

    Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
  • Tissue specificity

    Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bravo antibody
    • hBravo antibody
    • Neuronal cell adhesion molecule antibody
    • Neuronal surface protein Bravo antibody
    • Ng CAM related antibody
    • Ng-CAM-related antibody
    • NgCAM related cell adhesion molecule antibody
    • NgCAM-related cell adhesion molecule antibody
    • Nr CAM antibody
    • Nr-CAM antibody
    • Nrcam antibody
    • NRCAM_HUMAN antibody
    see all

Images

  • Anti-NrCAM antibody [EPR15654] (ab191418) at 1/50000 dilution + Human glioma lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 144 kDa

  • Western blot analysis of immunoprecipitation pellet from Human glioma lysate immunoprecipitated using ab191418 at 1/30 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

  • Anti-NrCAM antibody [EPR15654] (ab191418) at 1/10000 dilution + Human forebrain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 144 kDa

References

ab191418 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191418.
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