Anti-NrCAM antibody - Neuronal Marker (ab24344)
Key features and details
- Rabbit polyclonal to NrCAM - Neuronal Marker
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
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Overview
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Product name
Anti-NrCAM antibody - Neuronal Marker
See all NrCAM primary antibodies -
Description
Rabbit polyclonal to NrCAM - Neuronal Marker -
Host species
Rabbit -
Specificity
ab24344 immunoblots a single major protein of NrCAM (see western blot image on datasheet) and recognizes proteins of comparable size from mouse, rat and human. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse NrCAM. Mouse NrCAM protein as fusion with human Fc region of Ig. This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.
Database link: Q810U4 -
General notes
This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500.
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WB |
1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa).
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Notes |
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ICC/IF
1/500. |
WB
1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa). |
Target
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Function
Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier. -
Tissue specificity
Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 319504 Mouse
- SwissProt: Q810U4 Mouse
- Unigene: 208439 Mouse
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Alternative names
- Bravo antibody
- hBravo antibody
- Neuronal cell adhesion molecule antibody
see all
Images
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ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um
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Anti-NrCAM antibody - Neuronal Marker (ab24344) at 1/500 dilution + 20 µg of postnatal day 8 mouse brain extract in SDS
Predicted band size: 145 kDa
Observed band size: 145 kDa
ab24344 also can be used for immunoprecipatation and blocks function
Protocols
Datasheets and documents
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Datasheet download
References (38)
ab24344 has been referenced in 38 publications.
- Gorenberg EL et al. Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function. PLoS Biol 20:e3001590 (2022). PubMed: 35358180
- Zhou R et al. Proteomic and functional analyses of the periodic membrane skeleton in neurons. Nat Commun 13:3196 (2022). PubMed: 35680881
- Vilallongue N et al. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nat Commun 13:6040 (2022). PubMed: 36229455
- Takano T et al. Chemico-genetic discovery of astrocytic control of inhibition in vivo. Nature 588:296-302 (2020). PubMed: 33177716
- Rudan Njavro J et al. Mouse brain proteomics establishes MDGA1 and CACHD1 as in vivo substrates of the Alzheimer protease BACE1. FASEB J 34:2465-2482 (2020). PubMed: 31908000