Overview

  • Product name

  • Description

    Rabbit polyclonal to NRDP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Monkey, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human NRDP1 aa 47-232.
    Sequence:

    FSQQQTCPVDRSVV TVAHLRPVPR IMRNMLSKLQ IACDNAVFGC SAVVRLDNLM SHLSDCEHNP KRPVTCEQGC GLEMPKDELP NHNCIKHLRS VVQQQQTRIA ELEKTSAEHK HQLAEQKRDI QLLKAYMRAI RSVNPNLQNL EETIEYNEIL EWVNSLQPAR VTRWGGMIST PDAVLQAVIK RS

  • Positive control

    • A431 and 293T cells.
  • General notes

    Protein previously labeled as NRDP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab151231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88 and Negatively regulates MYD88-dependent production of proinflammatory cytokines but can promote TRIF-dependent production of type I interferon. Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (By similarity). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PARK2 modifier that accelerates its degradation, resulting in a reduction of PARK2 activity, influencing the balance of intracellular redox state.
  • Tissue specificity

    Detected in ovary, testis and prostate.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
    Contains 1 SIAH-type zinc finger.
  • Post-translational
    modifications

    Autoubiquitinated. Autoubiquitination leads to proteasomal degradation. Deubiquitinated by USP8 to get stabilized which induces apoptosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4930511A05Rik antibody
    • 4933415P08Rik antibody
    • E3 ubiquitin protein ligase NRDP1 antibody
    • E3 ubiquitin-protein ligase NRDP1 antibody
    • Fetal Liver RING finger protein antibody
    • FLRF antibody
    • MGC45228 antibody
    • Neuregulin receptor degradation protein 1 antibody
    • NRDP1 antibody
    • RING finger protein 41 antibody
    • rnf41 antibody
    • RNF41_HUMAN antibody
    • SBBI03 antibody
    see all

Images

  • Anti-NRDP1 antibody (ab151231) at 1/3000 dilution + A431 cell lysate at 30 µg

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling NRDP1 with ab151231 at 1/500 dilution. Botton panel is costained with Hoechst 33342.

References

ab151231 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate Cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na Citrate
Permeabilization
No
Specification
Prostate Cancer
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formalin

Mr. Salma Siddiqui

Verified customer

Submitted Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up