Product nameAnti-NRF1 antibody
See all NRF1 primary antibodies
DescriptionRabbit polyclonal to NRF1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
Recombinant fragment, corresponding to a region within amino acids 1-280 of Human NRF1
- Purchase matching WB positive control:Recombinant Human NRF1 protein
- 293T, A431, HeLa and HepG2 whole cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab154269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionTranscription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Tissue specificityUbiquitously expressed with strongest expression in skeletal muscle.
Sequence similaritiesBelongs to the NRF1/Ewg family.
modificationsPhosphorylation enhances DNA binding.
- Information by UniProt
- alpha pal antibody
- alpha palindromic binding protein antibody
- Alpha palindromic-binding protein antibody
All lanes : Anti-NRF1 antibody (ab154269) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
10% SDS PAGE
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling NRF1 with ab154269 at 1/500 dilution.