Anti-NRF1 antibody (ab34682)
Key features and details
- Rabbit polyclonal to NRF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NRF1 antibody
See all NRF1 primary antibodies -
Description
Rabbit polyclonal to NRF1 -
Host species
Rabbit -
Specificity
ab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Rat
Chicken
Dog
Chimpanzee
Zebrafish
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
|
| IHC-P |
Use a concentration of 2 - 5 µg/ml.
|
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands. |
|
IHC-P
Use a concentration of 2 - 5 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. -
Tissue specificity
Ubiquitously expressed with strongest expression in skeletal muscle. -
Sequence similarities
Belongs to the NRF1/Ewg family. -
Post-translational
modificationsPhosphorylation enhances DNA binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 416677 Chicken
- Entrez Gene: 4899 Human
- Entrez Gene: 312195 Rat
- Entrez Gene: 64604 Zebrafish
- Omim: 600879 Human
- SwissProt: Q16656 Human
- SwissProt: Q62792 Rat
- SwissProt: Q90X44 Zebrafish
see all -
Alternative names
- alpha pal antibody
- alpha palindromic binding protein antibody
- Alpha palindromic-binding protein antibody
see all
Images
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Western blot - Anti-NRF1 antibody (ab34682)This image is courtesy of an anonymous AbreviewAll lanes : Anti-NRF1 antibody (ab34682) at 1/1000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55,67 kDa why is the actual band size different from the predicted?
Exposure time: 50 seconds
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Western blot - Anti-NRF1 antibody (ab34682)All lanes : Anti-NRF1 antibody (ab34682) at 1/500 dilution
Lane 1 : HeLa Nuclear extract
Lane 2 : HeLa Whole Cell lysate
Secondary
All lanes : IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (ab34682)Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed. -
Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody (ab34682)ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab34682 has been referenced in 32 publications.
- Hou Z et al. Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1. Biochem J 476:1247-1266 (2019). PubMed: 30914440
- Wang X et al. Transcription regulation of NRF1 on StAR reduces testosterone synthesis in hypoxemic murine. J Steroid Biochem Mol Biol 191:105370 (2019). PubMed: 31028793
- Kim D et al. Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice. Behav Brain Res 374:112105 (2019). PubMed: 31325514
- Senapati P et al. Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer. Epigenetics Chromatin 12:44 (2019). PubMed: 31315653
- Le Berre G et al. Repression of TERRA Expression by Subtelomeric DNA Methylation Is Dependent on NRF1 Binding. Int J Mol Sci 20:N/A (2019). PubMed: 31181625