- Datasheet
- Specific References (19)
- Protocols
Overview
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Product nameAnti-NRF1 antibody
See all NRF1 primary antibodies -
DescriptionRabbit polyclonal to NRF1
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Host speciesRabbit
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Specificityab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal).
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Tested applicationsSuitable for: ChIP, WB, IHC-P, ICC/IF, ELISAmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Dog, Chimpanzee, Zebrafish
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1
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Positive control
- HeLa Whole Cell Lysate HeLa Cell Nuclear Extract Human testis
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab34682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands. | |
| IHC-P | Use a concentration of 2 - 5 µg/ml. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| ELISA | 1/3000 - 1/10000. | |
| EMSA | Use at an assay dependent concentration. PubMed: 21609478 |
Target
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FunctionTranscription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
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Tissue specificityUbiquitously expressed with strongest expression in skeletal muscle.
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Sequence similaritiesBelongs to the NRF1/Ewg family.
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Post-translational
modificationsPhosphorylation enhances DNA binding. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 416677 Chicken
- Entrez Gene: 4899 Human
- Entrez Gene: 18181 Mouse
- Entrez Gene: 312195 Rat
- Entrez Gene: 64604 Zebrafish
- Omim: 600879 Human
- SwissProt: Q16656 Human
- SwissProt: Q9WU00 Mouse
see all -
Alternative names
- alpha pal antibody
- alpha palindromic binding protein antibody
- Alpha palindromic-binding protein antibody
see all
Images
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Western blot - Anti-NRF1 antibody (ab34682)This image is courtesy of an anonymous AbreviewAll lanes : Anti-NRF1 antibody (ab34682) at 1/1000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55,67 kDa (why is the actual band size different from the predicted?)
Exposure time: 50 seconds
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Western blot - Anti-NRF1 antibody (ab34682)All lanes : Anti-NRF1 antibody (ab34682) at 1/500 dilution
Lane 1 : HeLa Nuclear extract
Lane 2 : HeLa Whole Cell lysate
Secondary
All lanes : IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 67 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (ab34682)Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed. -
Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody (ab34682)ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Dumont S et al. Transcriptional orchestration of mitochondrial homeostasis in a cellular model of PGC-1-related coactivator-dependent thyroid tumor. Oncotarget 9:15883-15894 (2018). ChIP ; Human . Read more (PubMed: 29662614) »
- Takiuchi Y et al. HTLV-1 bZIP factor suppresses TDP1 expression through inhibition of NRF-1 in adult T-cell leukemia. Sci Rep 7:12849 (2017). EMSA, ChIP . Read more (PubMed: 28993637) »
Customer reviews and Q&As
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