Overview

  • Product name

  • Description

    Rabbit polyclonal to NRF1
  • Host species

    Rabbit
  • Specificity

    ab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal).
  • Tested applications

    Suitable for: ChIP, WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Dog, Chimpanzee, Zebrafish
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1

  • Positive control

    • HeLa Whole Cell Lysate HeLa Cell Nuclear Extract Human testis

Properties

Applications

Our Abpromise guarantee covers the use of ab34682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
IHC-P Use a concentration of 2 - 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/3000 - 1/10000.
EMSA Use at an assay dependent concentration. PubMed: 21609478

Target

Images

  • All lanes : Anti-NRF1 antibody (ab34682) at 1/1000 dilution

    Lane 1 : Mouse liver whole tissue lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 55,67 kDa
    why is the actual band size different from the predicted?


    Exposure time: 50 seconds

    See Abreview

  • All lanes : Anti-NRF1 antibody (ab34682) at 1/500 dilution

    Lane 1 : HeLa Nuclear extract
    Lane 2 : HeLa Whole Cell lysate

    Secondary
    All lanes : IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution

    Predicted band size: 57 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?

  • Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed.
  • ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Hou Z  et al. Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1. Biochem J 476:1247-1266 (2019). Read more (PubMed: 30914440) »
  • Wang X  et al. Transcription regulation of NRF1 on StAR reduces testosterone synthesis in hypoxemic murine. J Steroid Biochem Mol Biol 191:105370 (2019). Read more (PubMed: 31028793) »
See all 26 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 19 2014

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver, muscle)
Loading amount
20 µg
Specification
liver, muscle
Gel Running Conditions
Reduced Denaturing (4-12% BioRad Criterion XT)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 26 2009

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
15 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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