For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    nrf1-antibody-epr5554n-chip-grade-ab175932.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Other
Share by email
RecombinantRabMAb

Recombinant Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

  • Datasheet
Reviews (2) Submit a question References (43)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ChIC/CUT&RUN sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • ChIP-sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
  • Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5554(N)] to NRF1 - ChIP Grade
  • Suitable for: Flow Cyt (Intra), ChIP, WB, ICC/IF, IP, IHC-P, ChIC/CUT&RUN-seq, ChIP-sequencing
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

Cocktail
Product image
Total OXPHOS Human WB Antibody Cocktail (ab110411)
Primary
Product image
Anti-Nrf2 antibody [EP1808Y] - BSA and Azide free (ab180845)
Protein
Product image
ChIC/CUT&RUN pAG-MNase (ab285373)

View more associated products

Overview

  • Product name

    Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade
    See all NRF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5554(N)] to NRF1 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ChIP, WB, ICC/IF, IP, IHC-P, ChIC/CUT&RUN-seq, ChIP-sequencingmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NRF1 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16656

  • Positive control

    • WB: MCF-7, HeLa and 293T cell lysates and human fetal heart, mouse heart, mouse brain, rat heart and rat brain tissue lysates. IHC-P: Human gastric adenocarcinoma, human cervical carcinoma and human skeletal muscle tissues. ICC/IF: HeLa and MCF-7 cells. Flow Cyt (intra): 293T cells. IP: 293T cell lysate. ChIP-Seq: HeLA cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5554(N)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-NRF1 antibody [EPR5554(N)] (ab200976)
    • Alexa Fluor® 647 Anti-NRF1 antibody [EPR5554(N)] (ab200979)
    • Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade – BSA and Azide free (ab221792)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse heart normal tissue lysate - total protein (ab30291)
  • Recombinant Protein

    • Recombinant Human NRF1 protein (ab132404)
  • Related Products

    • Recombinant Human NRF1 protein (ab132404)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP
Use at an assay dependent concentration.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ChIC/CUT&RUN-seq
Use 5µg for 105 cells.
ChIP-sequencing
Use 8µg for 107 cells.
Notes
Flow Cyt (Intra)
1/10 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ChIC/CUT&RUN-seq
Use 5µg for 105 cells.
ChIP-sequencing
Use 8µg for 107 cells.

Target

  • Function

    Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
  • Tissue specificity

    Ubiquitously expressed with strongest expression in skeletal muscle.
  • Sequence similarities

    Belongs to the NRF1/Ewg family.
  • Post-translational
    modifications

    Phosphorylation enhances DNA binding.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q16656 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4899 Human
    • Entrez Gene: 18181 Mouse
    • Entrez Gene: 312195 Rat
    • Omim: 600879 Human
    • SwissProt: Q16656 Human
    • SwissProt: Q9WU00 Mouse
    • SwissProt: Q62792 Rat
    • Unigene: 654363 Human
    • Unigene: 259258 Mouse
    • Unigene: 224754 Rat
    see all
  • Alternative names

    • alpha pal antibody
    • alpha palindromic binding protein antibody
    • Alpha palindromic-binding protein antibody
    • Alpha-pal antibody
    • locus control region factor 1 antibody
    • NFE2 related factor 1 antibody
    • NRF-1 antibody
    • Nrf1 antibody
    • NRF1_HUMAN antibody
    • Nuclear respiratory factor 1 antibody
    see all

Images

  • ChIC/CUT&RUN sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    ChIC/CUT&RUN sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373, 105 HeLa cells and 5µg of ab175932 [EPR5554(N)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The ChIP data was conducted on chromatin prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 µg of ab175932 [EPR5554(N)]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

    Additional screenshots of mapped reads can be downloaded here.

  • ChIP-sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    ChIP-sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 µg of ab175932 [EPR5554(N)]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

    Additional screenshots of mapped reads can be downloaded here.

  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    All lanes : Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932) at 1/5000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cevical carcinoma tissue labelling NRF1 with purified ab175932 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling NRF1 with purified ab175932 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Intracellular Flow Cytometry analysis of 293T cells labelling NRF1 with purified ab175932 at a dilution of 1/150 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Chromatin was prepared from Hela cells according to the Abcam Dual X-ChIP protocol. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab175932 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    ab175932 (purified) at a dilution of 1/50 immunoprecipitating NRF1 in 293T whole cell lysate.

    Lane 1 (input): 293T whole cell lysate (10µg)

    Lane 2 (+): ab175932 + 293T whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175932 in 293T whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Chromatin was prepared from NIH/3T3 treated with MG-132(2uM 16h) cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab175932 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932) at 1/10000 dilution (purified) + HEK293 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932) at 1/10000 dilution (purified) + HeLa whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Western blot - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    All lanes : Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932) at 1/1000 dilution (unpurified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Human fetal heart tissue lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling NRF1 with unpurified ab175932 at a dilution of 1/50.

  • Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    ab175932 (unpurified) at a dilution of 1/10 immunoprecipitating NRF1 in 293T cell lysate.

  • Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Intracellular flow cytometric analysis of permeabilized 293T cells labeling NRF1 with unpurified ab175932 at a dilution of 1/10 (red) compared to a negative control (rabbit IgG,green). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labeling NRF1 with unpurified ab175932 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric adenocarcinoma tissue labeling NRF1 with unpurified ab175932 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)
    Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (ab175932)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (43)

Publishing research using ab175932? Please let us know so that we can cite the reference in this datasheet.

ab175932 has been referenced in 43 publications.

  • Wang B  et al. The positive regulation loop between NRF1 and NONO-TFE3 fusion promotes phase separation and aggregation of NONO-TFE3 in NONO-TFE3 tRCC. Int J Biol Macromol 176:437-447 (2021). PubMed: 33592266
  • Wang X  et al. Testosterone attenuates pulmonary epithelial inflammation in male rats of COPD model through preventing NRF1-derived NF-?B signaling. J Mol Cell Biol 13:128-140 (2021). PubMed: 33475136
  • Al-Obaide MAI  et al. Unexplored regulatory sequences of divergently paired GLA and HNRNPH2 loci pertinent to Fabry disease in human kidney and skin cells: Presence of an active bidirectional promoter. Exp Ther Med 21:154 (2021). PubMed: 33456521
  • Song J  et al. Qiangji Jianli Decoction Alleviates Hydrogen Peroxide-Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts. Oxid Med Cell Longev 2021:6660616 (2021). PubMed: 33936383
  • Nisr RB  et al. Mono- and Polyunsaturated Fatty Acids Counter Palmitate-Induced Mitochondrial Dysfunction in Rat Skeletal Muscle Cells. Cell Physiol Biochem 54:975-993 (2020). PubMed: 32997464
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-NRF1 antibody [EPR5554(N)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 09 2016

Western blot abreview for Anti-NRF1 antibody [EPR5554(N)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12% tris-glycine gel)
Sample
Human Tissue lysate - whole (heart)
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 10 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.