Product nameAnti-Nrf2 antibody
See all Nrf2 primary antibodies
DescriptionRabbit polyclonal to Nrf2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Nrf2 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q60795
- This antibody gave a positive signal in the following Mouse tissue lysates: Liver; Skeletal Muscle; Heart; Lung; Kidney.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Our Abpromise guarantee covers the use of ab156883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 68 kDa).|
FunctionTranscription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Tissue specificityWidely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.
DomainAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
modificationsPhosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
- Information by UniProt
- erythroid derived 2 antibody
- HEBP1 antibody
- like 2 antibody
All lanes : Anti-Nrf2 antibody (ab156883) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lane 5 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156883 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.