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I did WB with the Ab for Nrf2(ab31163, Lot #GR78857-3) which is easy to be ubiquitinated. From the primary cultured cardiac myocytes from rats, I got 3 bands at around 100Kda, 68Kda and 30Kda. Do you think I can considered 100Kda proteins as ubiquitinated Nrf2?
Asked on Oct 30 2012