Product nameAnti-Nrf2 antibody
See all Nrf2 primary antibodies
DescriptionRabbit polyclonal to Nrf2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Nrf2 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, Ramos, A498, Jurkat - Staurosporine Treated (24hr, 500nM), SK N SH
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab71890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Nrf2 peptide (ab71889).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionTranscription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Tissue specificityWidely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.
DomainAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
modificationsPhosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
- Information by UniProt
- erythroid derived 2 antibody
- HEBP1 antibody
- like 2 antibody
All lanes : Anti-Nrf2 antibody (ab71890) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 5 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
The band observed at 90 kDa is consistent with the banding pattern observed for other commercially available antibodies to Nrf2 and is thought to correspond to the polyubiquinated form of Nrf2.
ICC/IF image of ab71890 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.