Overview

  • Product name
  • Description
    Rabbit polyclonal to Nrf2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human
    Predicted to work with: Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    SQRRKEYELEKQKKLEKERQE

    conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 40-60 of Human Nrf2

  • Positive control
    • Rat liver microsomes, Mouse liver microsomes, Human liver microsomes, Canine liver microsomes and Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab92946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).

Target

  • Function
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • Tissue specificity
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • Sequence similarities
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • Domain
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • Post-translational
    modifications
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Images

  • All lanes : Anti-Nrf2 antibody (ab92946) at 1/1000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Rat liver microsomes
    Lane 3 : Mouse liver microsomes
    Lane 4 : Human liver microsomes
    Lane 5 : Canine liver microsomes
    Lane 6 : HeLa cell lysate

    Developed using the ECL technique.

    Predicted band size: 70 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Wen ZS  et al. Protective effect of low molecular-weight seleno-aminopolysaccharides against H2O2-induecd oxidative stress in intestinal epithelial cells. Int J Biol Macromol 112:745-753 (2018). WB . Read more (PubMed: 29410059) »
  • Rabbani PS  et al. Targeted Nrf2 activation therapy with RTA 408 enhances regenerative capacity of diabetic wounds. Diabetes Res Clin Pract 139:11-23 (2018). WB ; Mouse . Read more (PubMed: 29476889) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab92946 has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new information i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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