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RecombinantRabMAb

Recombinant Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

  • Datasheet
  • SDS
Reviews (16)Q&A (14)References (110)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Flow Cytometry - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
  • Suitable for: Dot blot, ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

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Secondary
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View more associated products

Overview

  • Product name

    Anti-Nrf2 (phospho S40) antibody [EP1809Y]
    See all Nrf2 primary antibodies
  • Description

    Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, ICC/IF, IHC-P, WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Nrf2 (phospho S40). The exact sequence is proprietary.
    (Peptide available as ab133404)

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900). IHC-P: Human tonsil, breast carcinoma, ovarian carcinoma and cervical carcinoma tissue. ICC/IF: HepG2 cells. Flow Cyt: Jurkat cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1809Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
    • Alexa Fluor® 488 Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab237464)
    • Alexa Fluor® 647 Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab237465)
  • Assay kits

    • Nrf2 Transcription Factor Assay Kit (Colorimetric) (ab207223)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Nrf2 (phospho S40) peptide (ab133404)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • Hep G2 nuclear extract lysate (ab14660)
  • Recombinant Protein

    • Recombinant Human Nrf2 protein (ab132356)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
ICC/IF (1)
1/100 - 1/250.
IHC-P (3)
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB (11)
1/5000 - 1/50000. Predicted molecular weight: 68 kDa.Can be blocked with Human Nrf2 (phospho S40) peptide (ab133404).
Flow Cyt
1/80 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
1/5000 - 1/50000. Predicted molecular weight: 68 kDa.Can be blocked with Human Nrf2 (phospho S40) peptide (ab133404).
Flow Cyt
1/80 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
    • Tissue specificity

      Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
    • Sequence similarities

      Belongs to the bZIP family. CNC subfamily.
      Contains 1 bZIP domain.
    • Domain

      Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
    • Post-translational
      modifications

      Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
    • Target information above from: UniProt accession Q16236 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4780 Human
      • Omim: 600492 Human
      • SwissProt: Q16236 Human
      • Unigene: 744006 Human
      • Alternative names

        • erythroid derived 2 antibody
        • HEBP1 antibody
        • like 2 antibody
        • NF E2 related factor 2 antibody
        • NF-E2-related factor 2 antibody
        • NF2L2_HUMAN antibody
        • NFE2 related factor 2 antibody
        • NFE2-related factor 2 antibody
        • Nfe2l2 antibody
        • Nrf 2 antibody
        • NRF2 antibody
        • Nuclear factor (erythroid derived 2) like 2 antibody
        • Nuclear factor antibody
        • nuclear factor erythroid 2 like 2 antibody
        • Nuclear factor erythroid 2 related factor 2 antibody
        • Nuclear factor erythroid 2-related factor 2 antibody
        • Nuclear factor erythroid derived 2 like 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)Inui et al PLoS One. 2013 Sep 24;8(9):e74398. doi: 10.1371/journal.pone.0074398. eCollection 2013. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Nrf2 was abundantly expressed in carcinomas, low grade dysplasias, and non-atypical epithelia of oral tissue.

        Representative findings of Nrf2 staining in carcinoma (left), in low grade dysplasia (middle), and in non-atypical epithelium (right).

        Corresponding PLA signals are displayed in the lower row. Scale bar; 100 µm.

        Surgical specimens were transferred to 10% buffered formalin and fixed overnight. The fixed samples were embedded in paraffin, and serially sliced into 5 µm sections. After dewaxing, sections were autoclaved at 120°C for 1 min in 10 mM sodium citrate buffer (pH 6.0), and immersed in 0.3% H2O2. They were then incubated overnight at 4°C with primary antibody to Nrf2 (diluted 1:200). The sections were rinsed with 1×PBS and incubated with the secondary antibody conjugated with horseradish peroxidase at room temperature for 1 hour. The sections were then stained with 3.3′-diaminobenzidinetetrahydrochloride (DAB) and counterstained with hematoxylin.

      • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)Giyshi et al PLoS Pathog. 2014 Oct 23;10(10):e1004460. doi: 10.1371/journal.ppat.1004460. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Immunofluorescence analysis of Nrf2 levels in Kaposi's sarcoma skin lesions.

        B) Healthy skin (top two rows) and KS skin tissue (bottom row) were double-stained for LANA-1 (Alexa-Fluor 594- red) and host phosphorylated pNrf2 (ab76026) (Alexa-Fluor®488 – green). DAPI was used to visualize the nuclei, and the triple merge of LANA-1, pNrf2 and DAPI is shown in the third column.

        Yellow square = enlarged area.

      • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)Masuda et al PLoS One. 2015 Jun 25;10(6):e0131012. doi: 10.1371/journal.pone.0131012. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Nrf2 Translocation from cytoplasm to nucleus.

        (A) Human islets were treated with dh404 for 0.5, 1 or 2 hours. The treated and untreated samples were stained with Nrf2 antibody ab76026 (Green) and DAPI (Blue). The con-focal microscope clearly showed that the Nrf2 translocation from cytoplasm to nucleus in the dh404 treated human islet cells

      • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/50000 dilution (purified)

        Lane 1 : untreated HepG2 cell lysate
        Lane 2 : HepG2 treated with phosphatase lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

        Predicted band size: 68 kDa
        Observed band size: 90 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab76026 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
      • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Immunofluorescence staining of HepG2 cells with purified ab76026 at a working dilution of 1/100, counter-stained with DAPI. The treated cells were treated with alkaline phosphatase for 1 h at 37°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab76026 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      • Flow Cytometry - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Flow Cytometry - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab76026 at a dilution of 1 in 80 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
      • Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Dot blot analysis of Nrf2 peptides using unpurified ab76026 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

         

        Lane 1: Nrf2 (pS40) phospho peptide

        Lane 2: Nrf2 non-phospho peptide

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Immunohistochemical analysis of paraffin-embedded human breast carcinoma using unpurified ab76026 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/10000 dilution (unpurified)

        Lane 1 : Untreated HepG2 (human hepatocellular carcinoma) whole cell lysates 20µg
        Lane 2 : HepG2 (human hepatocellular carcinoma) treated with Alkaline Phosphatase (AP) whole cell lysates 20µg.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

        Predicted band size: 68 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST, dilution buffer: 5% NFDM /TBST, exposure time: 15 seconds

      • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution (unpurified)

        Lane 1 : HepG2 cell lysate
        Lane 2 : HepG2 cell lysate treated with AP

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

        Predicted band size: 68 kDa
        Observed band size: 90 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)This image is courtesy of an anonymous Abreview

        Unpurified ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.

        See Abreview

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Unpurified ab76026 showing positive staining in Breast carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Unpurified ab76026 showing positive staining in Cervical carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Unpurified ab76026 showing positive staining in Ovarian carcinoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

        Unpurified ab76026 showing positive staining in Normal tonsil tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
        Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (110)

      Publishing research using ab76026? Please let us know so that we can cite the reference in this datasheet.

      ab76026 has been referenced in 110 publications.

      • Jegal KH  et al. Luteolin prevents liver from tunicamycin-induced endoplasmic reticulum stress via nuclear factor erythroid 2-related factor 2-dependent sestrin 2 induction. Toxicol Appl Pharmacol 399:115036 (2020). PubMed: 32407927
      • Jie Z  et al. Nrf2 modulates immunosuppressive ability and cellular senescence of human umbilical cord mesenchymal stem cells. Biochem Biophys Res Commun 526:1021-1027 (2020). PubMed: 32317184
      • Patra U  et al. Progressive Rotavirus Infection Downregulates Redox-Sensitive Transcription Factor Nrf2 and Nrf2-Driven Transcription Units. Oxid Med Cell Longev 2020:7289120 (2020). PubMed: 32322337
      • Zhang Y  et al. Rutaecarpine inhibits KEAP1-NRF2 interaction to activate NRF2 and ameliorate dextran sulfate sodium-induced colitis. Free Radic Biol Med 148:33-41 (2020). PubMed: 31874248
      • Lin CC  et al. Induction of HO-1 by Mevastatin Mediated via a Nox/ROS-Dependent c-Src/PDGFRa/PI3K/Akt/Nrf2/ARE Cascade Suppresses TNF-a-Induced Lung Inflammation. J Clin Med 9:N/A (2020). PubMed: 31952230
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 30 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (SH-SY5YH)
      Permeabilization
      Yes - TRITON X-100 0.05% in PBS
      Specification
      SH-SY5YH
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted May 22 2020

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (SH-SY5Y cells)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      30 µg
      Treatment
      H2O2 50uM for 24 hrs
      Specification
      SH-SY5Y cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 13 2020

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Primary Prostate Fibroblasts)
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Loading amount
      70 µg
      Treatment
      SOD
      Specification
      Primary Prostate Fibroblasts
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Elizabeth Kosmacek

      Verified customer

      Submitted Oct 25 2019

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (BV2 microglia)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      70 µg
      Treatment
      5µM LPA for different time points
      Specification
      BV2 microglia
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Dr. Lisha Joshi

      Verified customer

      Submitted Jun 12 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (pancreas)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10 mM TRIS-EDTA pH 9 at 98°C for 20 minutes, normal pressure
      Permeabilization
      Yes - 0.04% triton 100 x in the blocking solution
      Specification
      pancreas
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 13 2016

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (lungs)
      Gel Running Conditions
      Reduced Denaturing (30 minutes RT, 10% gel)
      Loading amount
      100 µg
      Specification
      lungs
      Blocking step
      2 % Topblock in TBT-0.1% Triton 100 x as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 13 2016

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (brain)
      Gel Running Conditions
      Reduced Denaturing (10)
      Loading amount
      50 µg
      Specification
      brain
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 12 2016

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Brain)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      50 µg
      Specification
      Brain
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 12 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (breast cancer cell line xenograft)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
      Permeabilization
      No
      Specification
      breast cancer cell line xenograft
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 17 2015

      Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human Placenta)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      30 µg
      Specification
      Human Placenta
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Vicky

      Verified customer

      Submitted Nov 13 2015

      1-10 of 30 Abreviews or Q&A

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