Recombinant
RabMAb

Recombinant Anti-NRG3 antibody [EPR4148] - BSA and Azide free (ab232014)

Overview

  • Product name

    Anti-NRG3 antibody [EPR4148] - BSA and Azide free
    See all NRG3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4148] to NRG3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NRG3 aa 650-750 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Flow Cytometry: Permeabilized C6 cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232014 is a PBS-only buffer format of ab109256. Please refer to ab109256 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.

Target

  • Function

    Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
  • Tissue specificity

    Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the neuregulin family.
    Contains 1 EGF-like domain.
  • Developmental stage

    Isoform 3 is expressed in fetal brain but not in other fetal tissues.
  • Domain

    The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • Post-translational
    modifications

    Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
  • Cellular localization

    Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
  • Information by UniProt
  • Database links

  • Alternative names

    • HRG3 antibody
    • Neuregulin 3 antibody
    • Neuregulin 3 like polypeptide antibody
    • Neuregulin-3 antibody
    • Neuregulin3 antibody
    • NRG-3 antibody
    • Nrg3 antibody
    • NRG3_HUMAN antibody
    • Pro neuregulin 3 antibody
    • pro NRG3 antibody
    • Pro-NRG3 antibody
    see all

Images

  • Flow cytometric analysis of permeabilized C6 (Rat glial tumor cell line) cells using ab109256 (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109256).

References

ab232014 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232014.
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